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US 11,136,569 B2Granted
Heat resistant isoamylase
AssigneeGodo Shusei Co., Ltd.
InventorsMasahiro Baba, Ryoko Sano, Shun Ogawa, Hirofumi Horiguchi, Jun Yoshikawa
Life of this patent
Estimating the term…
Abstract
Provided are a novel isoamylase improved in optimum temperature, and more specifically, improved in heat resistance, and a process for producing the isoamylase. An isoamylase having at least one amino acid mutation selected from the group consisting of D268A, M277I, A549P, A554P and A580T in an isoamylase consisting of an amino acid sequence represented by SEQ ID No: 1 or an isoamylase consisting of the amino acid sequence represented by SEQ ID No: 1 and having deletion, substitution or insertion of one to several amino acid residues.
Claims6
An isoamylase, comprising:
- a double to quintuple mutation selected from the group consisting of D268A, M277I, A549P, A554P and A580T having isoamylase activity in an isoamylase consisting of the amino acid sequence of SEQ ID NO:1, or an isoamylase consisting of the amino acid sequence with at least 90% identity to SEQ ID NO:1 and comprising a double to quintuple mutation selected from the group consisting of D268A, M277I, A549P, A554P and A580T having isoamylase activity.
Dependencies are inferred from the claim text.