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US 11,136,559 B2Granted
Engineered prephenate dehydrogenases and arogenate dehydrogenases and methods of using the same
AssigneeWisconsin Alumni Research Foundation
InventorsHiroshi A. Maeda, Craig Albert Schenck
Life of this patent
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Abstract
The invention generally relates to engineered prephenate dehydrogenases and arogenate dehydrogenases and methods of using the same. More specifically, the invention relates in part to compositions including engineered prephenate dehydrogenases (PDH) polypeptides and engineered arogenate dehydrogenase (ADH) polypeptides with altered substrate preferences and tyrosine sensitivities and methods of using the same.
Claims11
An engineered arogenate dehydrogenase polypeptide comprising at least 95% sequence identity to any one of the polypeptides of SEQ ID NOs:
- 10-55 having a non-acidic amino acid at a position corresponding to residue 220 of SEQ ID NO:10, SEQ ID NOs:124-143 having a non-acidic amino acid at a position corresponding to residue 220 of SEQ ID NO:10, SEQ ID NOs:149-151 having a non-acidic amino acid at a position corresponding to residue 220 of SEQ ID NO:10, SEQ ID NOs:201-212 having a non-acidic amino acid at a position corresponding to residue 220 of SEQ ID NO:10, or SEQ ID NOs:218-242 having a non-acidic amino acid at a position corresponding to residue 220 of SEQ ID NO:10, wherein the engineered mutant arogenate dehydrogenase polypeptide has increased prephenate dehydrogenase activity as compared to an identical sequence with a D or E amino acid at a position corresponding to amino acid residue 220 of SEQ ID NO:
- 10.
Dependencies are inferred from the claim text.