Spot array substrate, method for producing same, and nucleic acid polymer analysis method and device

A spot array substrate comprising:

• a resin substrate having a surface including an uneven pattern that includes a plurality of grooves that form rhombic patterns, and a plurality of bead sitting positions set in a two-dimensional array within the uneven pattern;
• and
• a plurality of surface-modified beads loaded into the bead sitting positions of the resin substrate,
• wherein a thermal expansion coefficient of a material of the resin substrate is in a range from 10−5 to 10−4/° C.,
• wherein the plurality of bead sitting positions are intersections of the plurality of grooves disposed in the resin substrate,
• wherein an epoxy group is disposed as a film only on inner surfaces of the respective plurality of grooves of the resin substrate,
• wherein the respective surface-modified beads loaded in the bead sitting positions are physically clamped by inner surfaces of the grooves of the bead sitting positions, which are physically contracted, and
• wherein the surface-modified beads are at least one of functional group surface-modified beads and oligo DNA surface-modified beads.