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US 11,130,979 B2Granted
Protein expression strains
AssigneeAlbumedix Ltd
InventorsChristopher John Arthur Finnis, Per Kristoffer Nordeide, Jennifer Mary McLaughlan
Life of this patent
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Abstract
The invention provides an improved host strain for production of desired protein.
Claims42
A fungal host cell having:
- a. a reduced level of Gsh1 protein, and/or
- b. a reduced activity level and/or expression level of Gsh1 protein, and/or
- c. a reduced level of GSH1 gene, and/or
- d. a reduced level of expression of GSH1 gene and
- a nucleotide sequence encoding a heterologous protein wherein the heterologous protein is a serum protein or an albumin protein, and
- wherein the reduced level is relative to the level of a reference fungal host cell identical to the host cell with the exception of the GSH1 gene and/or Gsh1 protein, wherein the GSH1 gene of the reference encodes wild-type Gsh1 protein and/or the Gsh1 protein of the reference is wild-type Gsh1.
3 more elements
A method for increasing the yield of a heterologous protein comprising culturing a fungal host cell to produce the heterologous protein, wherein the fungal host cell has
- 1. a reduced level of Gsh1 protein, and/or
- 2. a reduced level of activity of Gsh1 protein, and/or
- 3. a reduced level of GSH1 gene, and/or
- 4. a reduced level of expression (preferably reduced) of GSH1 gene, and
- a nucleotide sequence encoding the heterologous protein, wherein the heterologous protein is a serum protein or an albumin protein, and
- wherein the reduced level is relative to the level of a reference fungal host cell identical to the fungal host cell with the exception of the GSH1 gene and/or Gsh1 protein, wherein the GSH1 gene of the reference fungal host cell encodes wild-type Gsh1 protein and/or the Gsh1 protein of the reference fungal host cell is wild-type Gsh1.
3 more elements
Dependencies are inferred from the claim text.