Method for organizing individual molecules on a patterned substrate and structures assembled thereby

A structure comprising:

• a substrate having a patterned surface of one or more negatively-charged binding sites and a non-binding background;
• and
• a molecular shape comprising polynucleotides generated by a polynucleotide platform, the molecular shape being bound to one of the one or more binding sites,
• wherein the molecular shape comprises a plurality of faces comprising a binding face and one or more non-binding faces,
• wherein the binding face of the molecular shape has an outline that matches the outline of the one or more binding sites,
• wherein the non-binding face of the molecular shape comprises a plurality of modifications that are repellant to the binding site,
• wherein the majority of the binding face of the molecular shape has an unmodified phosphate backbone, and
• wherein, in the presence of a multivalent cation, the one or more binding sites have a higher binding affinity for the unmodified phosphate backbone of the binding face than for the one or more non-binding faces.