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US 11,124,826 B2Granted
Kits comprising nucleic acid templates and primers for use in pre-amplification assays
AssigneeBio-Rad Laboratories, Inc.
InventorsYanhong Kong, Steven Okino
Life of this patent
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Abstract
Provided herein are methods and compositions to determine the efficacy of a nucleic acid pre-amplification reaction.
Claims16
A kit comprising:
- a first container containing a first nucleic acid template, a second nucleic acid template, and a set of primers specific for the first nucleic acid template, such that when the mixture in the first container is exposed to amplification conditions, a first nucleic acid template amplification product, but not a second nucleic acid template amplification product is formed;
- and
- a second container containing a set of primers specific for the second nucleic acid template, and a set of primers specific for the first nucleic acid template amplification product,
- wherein:
- (i) at least one of the primers in one of the sets is labeled, (ii) the kit comprises an intercalating dye, or (iii) the kit comprises a first labeled probe specific for an amplification product of the first nucleic acid template and/or a second labeled probe specific for an amplification product of the second nucleic acid template.
2 more elements
Dependencies are inferred from the claim text.