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US 11,124,820 B2Granted
Sample processing method and sample culturing method
AssigneeOlympus Corporation
InventorKatsunori Ogo
Life of this patent
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Abstract
Provided is a sample processing method that liquefies a medium solution by making a liquid that liquefies the medium solution act on a sample formed by gelating or solidifying the medium solution that is supported by a substrate while an observation subject is included therein, while maintaining a state in which the medium solution is supported by the substrate while the observation subject is included therein.
Claims12
A sample processing method comprising:
- liquefying at least part of a sample which is supported on a substrate as a hanging droplet and comprises a medium that contains alginate and is at least partially gelled or solidified, by causing a liquid comprising an alginate lyase or a chelating agent to act on the at least partially gelled or solidified medium,
- wherein the sample includes an observation subject therein, and
- wherein the substrate that supports the sample has a shape such that after the medium is at least partially liquefied, a resulting medium solution having a droplet shape remains supported as the hanging droplet by the substrate.
A sample culturing method comprising:
- at least partially gelating or solidifying a sample that is supported on a substrate as a hanging droplet and includes an observation subject therein, to obtain an at least partially gelled or solidified medium that contains alginate, is substantially transparent, is supported on the substrate as the hanging droplet, and includes the observation subject therein;
- observing the observation subject that is included in the medium;
- and
- after performing observation, at least partially liquefying the medium by causing a liquid comprising an alginate lyase or a chelating agent to act on the at least partially gelled or solidified medium,
- wherein the substrate that supports the sample has a shape such that after the medium is at least partially liquefied, a resulting medium solution having a droplet shape remains supported as the hanging droplet by the substrate.
2 more elements
Dependencies are inferred from the claim text.