Method for modifying microcystins and nodularins
AssigneeCyano Biotech GmbH
InventorsHeike Enke, Wolfram Lorenzen, Stefan Jahns, Dan Enke, Timo Niedermeyer, Julia Moschny
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Abstract
A method is used for producing a modified non-ribosomal peptide, e.g. a modified microcystin and/or modified nodularin (together CA), including the steps of: a) growing a modified non-ribosomal peptide producing cyanobacteria strain in a culture media, h) adding one or more modified substrates, preferably modified amino acids to said culture, and c) inoculating the non-ribosomal peptide, producing strain the presence of said modified substrates. The thus modified non-ribosomal peptide may be used for the therapy of various diseases.
Claims22
A method of producing a modified non-ribosomal peptide from cyanobacteria, comprising:
- a) growing a non-ribosomal peptide producing cyanobacteria strain in a culture medium,
- b) adding one or more modified substrates to said culture medium, and
- c) growing the cyanobacteria strain in the presence of said one or more modified substrates,
- wherein the one or more modified substrates are a modified amino acid that is not found in nature in the non-ribosomal peptide produced by said cyanobacteria strain, which comprises an anchor group that is directly accessible or transformable for use in conjugation chemistry for the attachment of a targeting moiety or a label.
Dependencies are inferred from the claim text.