Production of oxalyl-CoA, glyoxylate and/or glycolic acid
AssigneeTeknologian tutkimuskeskus VTT Oy
InventorsMervi Toivari, Marja Ilmén, Merja Penttilä
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Abstract
The present invention relates to a method of converting oxalate to oxalyl-coA and/or oxalyl-coA to glyoxylate in a fungus and to a method of producing glycolic acid. Still, the present invention relates to a genetically modified fungus comprising increased enzyme activity associated with oxalyl-CoA. And furthermore, the present invention relates to use of the fungus of the present invention for producing oxalate, oxalyl-coA, glyoxylate and/or glycolic acid from a carbon substrate. Still furthermore, the present invention relates to a method of producing specific products and to a method of preparing the genetically modified fungus of the present invention.
Claims21
A method of producing glycolic acid, said method comprising:
- providing a fungus that has been genetically modified to express or overexpress a gene encoding oxalyl-CoA ligase, oxalyl-CoA reductase and/or ketopantoate reductase;
- and
- culturing said fungus in a carbon substrate containing medium to obtain glycolic acid.
A method of converting oxalate to oxalyl-coA and/or oxalyl-coA to glyoxylate in a fungus, said method comprising:
- providing a fungus that has been genetically modified to express or overexpress a gene encoding oxalyl-CoA ligase, oxalyl-CoA reductase and/or ketopantoate reductase;
- and
- culturing said fungus in a carbon substrate containing medium to obtain oxalyl-coA and/or glyoxylate.
A genetically modified fungus comprising increased enzyme activity associated with oxalyl-CoA, as compared to a genetically unmodified fungus, wherein the enzyme activity associated with oxalyl-CoA is oxalyl-CoA ligase activity, oxalyl-CoA reductase activity or ketopantoate reductase activity, or any combination thereof.
Dependencies are inferred from the claim text.