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US 11,124,808 B2Granted
Solubilization of MSW with blend enzymes
AssigneeRenescience A/S
InventorsHanne Risbjerg Soerensen, Lisa Rosgaard, Henrik B. Nielsen, Lone Baekgaard, Joanna Wawrzynczyk
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Abstract
The present invention relates to a method for solubilisation or hydrolysis of Municipal Solid Waste (MSW) with an enzyme blend and an enzyme composition for solubilization of Municipal Solid Waste (MSW), the enzyme composition comprising a cellulolytic background composition and a protease, lipase and/or beta-glucanase.
Claims21
A process for solubilizing Municipal Solid Waste (MSW) comprising:
- (a) contacting the MSW with an enzyme composition comprising a cellulolytic background composition present at a ratio between 40-99% w/w of the total enzymes protein, a lipase present at a ratio between 2.5-7.5% w/w of the total enzymes protein, wherein the lipase is derived from Thermomyces lanuginosus, or wherein the lipase is a triacylglycerol lipase derived from Humicola insolens;
- and one or more enzymes selected from
- (i) a protease present at a ratio between 0-20% w/w of the total enzymes protein, wherein the protease is derived from Bacillus amyloliquefaciens;
- and
- (ii) a beta-glucanase present at a ratio between 0-30% w/w of the total enzymes protein, wherein the beta-glucanase is derived from Aspergillus aculeatus,
- in order to produce solubilized and/or hydrolysed MSW.
3 more elements
A process for solubilizing Municipal Solid Waste (MSW) comprising:
- (a) contacting the MSW with an enzyme composition comprising a cellulolytic background composition present at a ratio between 40-99% w/w of the total enzymes protein in combination with
- (i) a protease present at a ratio between 0-20% w/w of the total enzymes protein, wherein the amino acid sequence of the protease has at least 90% sequence identity to SEQ ID NO:
- 1;
- (ii) a lipase present at a ratio between 2.5-7.5% w/w of the total enzymes protein, where in the amino acid sequence of the lipase has at least 90% sequence identity to SEQ ID NO:
- 2 or SEQ ID NO:
- 3;
- and
- (iii) a beta-glucanase present at a ratio between 0-30% w/w of the total enzymes protein, wherein the amino acid sequence of the beta-glucanase has at least 90% sequence identity to SEQ ID NO:
- 4,
- in order to produce solubilized and/or hydrolysed MSW.
7 more elements
Dependencies are inferred from the claim text.