Sesquiterpene synthases for production of drimenol and mixtures thereof
AssigneeFirmenich SA
InventorsPan Li, Qi Wang, Xiu-Feng He, Olivier Haefliger
Life of this patent
Estimating the term…
Abstract
Described herein is a method of producing drimenol and/or drimenol derivatives, the method including contacting at least one polypeptide with farnesyl diphosphate (FPP). The method may be performed in vitro or in vivo. Also described herein are amino acid sequences of polypeptides useful in the methods and nucleic acids encoding the polypeptides described. Also described herein are host cells or organisms genetically modified to express the polypeptides and useful to produce drimenol and/or derivatives of drimenol.
Claims10
A method for producing drimenol or a mixture comprising drimenol comprising:
- a. transforming a host cell or non-human host organism with a nucleic acid encoding a polypeptide having drimenol synthase activity, wherein the polypeptide comprises an amino acid sequence having at least 95% sequence identity to the amino acid sequence of SEQ ID NO:
- 2 or comprises the amino acid sequence of SEQ ID NO:
- 2;
- b. contacting an acyclic farnesyl diphosphate (FPP) precursor with the polypeptide to produce drimenol or a mixture comprising drimenol and one or more terpenes;
- and
- c. optionally isolating the drimenol;
- wherein
- the host cell or non-human host organism is a plant, a prokaryote, a fungus, or a microorganism.
A method of using a vector comprising an isolated nucleic acid molecule comprising a nucleotide sequence selected from the group consisting of:
- a. a nucleotide sequence encoding an isolated polypeptide having drimenol synthase activity comprising an amino acid sequence having at least 95% sequence identity to the amino acid sequence of SEQ ID NO:
- 2 or comprising the amino acid sequence of SEQ ID NO:
- 2;
- b. a nucleotide sequence having at least 95% sequence identity to the nucleotide sequence of SEQ ID NO:
- 1 or SEQ ID NO:
- 3 and encoding an isolated polypeptide having drimenol synthase activity;
- and
- c. the nucleotide sequence of SEQ ID NO:
- 1 or SEQ ID NO:
- 3,
- the method comprising transforming a host cell or a non-human organism with the vector and producing drimenol or a mixture comprising drimenol and one or more terpenes;
- wherein the host cell or the non-human organism is a plant, a prokaryote, a fungus, or a microorganism.
A method of using a vector comprising a nucleic acid encoding a polypeptide having drimenol synthase activity comprising an amino acid sequence having at least 95% sequence identity to the amino acid sequence of SEQ ID NO:
- 2 or comprising the amino acid sequence of SEQ ID NO:
- 2, the method comprising transforming a host cell or a non-human organism with the vector and producing drimenol or a mixture comprising drimenol and one or more terpenes;
- wherein
- the host cell or the non-human organism is a plant, a prokaryote, a fungus, or a microorganism.
Dependencies are inferred from the claim text.