Fusion polypeptides comprising one or more inclusion body tags, methods and uses
AssigneeAbera Bioscience AB
InventorsWouter Simon Petrus Jong, Joen Luirink
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Abstract
A fusion polypeptide, comprising an inclusion body tag fused to at least one polypeptide of interest, is provided. The inclusion body tag comprises an inclusion body forming (IBF) amino acid sequence selected from X14X15AX17X18X19GLTVA GMLG and sequences having at least 71% identity thereto. Also provided are inclusion bodies comprising the fusion polypeptide, as well as nucleic acids, expression vectors, host cells and methods for its production.
Claims20
A fusion polypeptide comprising an inclusion body tag fused to at least one polypeptide of interest, wherein said inclusion body tag comprises an inclusion body forming (IBF) amino acid sequence selected from:
- i) X14X15AX17X18 X19GLTVAGMLG (SEQ ID NO:1)
- wherein, independently from each other,
- X14 is selected from F and Y;
- X15 is selected from L and M;
- X17 is selected from Q and E;
- X18 is selected from L, V and I;
- and
- X19 is selected from G and V;
- and
- ii) an amino acid sequence which has at least 71% identity to the sequence defined in i);
- wherein:
- (a) the fusion polypeptide comprises two or more of the inclusion body tags;
- (b) the inclusion body tag is C-terminal to said polypeptide of interest;
- or
- (c) the inclusion body tag comprises the amino acid sequence of SEQ ID NO:
- 3 or SEQ ID NO:44 lacking one or more of the amino acids corresponding to positions 29-36 of SEQ ID NO:
- 3 or SEQ ID NO:44.
Dependencies are inferred from the claim text.