Non-hydrogen-bonding universal reader for DNA sequencing
AssigneeArizona Board of Regents on Behalf of Arizona
InventorsPeiming Zhang, Sovan Biswas, Suman Sen, Stuart Lindsay
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Abstract
The present disclosure provides apparatus and methods for determining the sequence of a nucleic acid. The apparatus comprises electrodes that form a tunnel gap through which the nucleic acid can pass. The electrodes comprise a reagent that is capable of selectively interacting with a nucleobase of the nucleic acid sequence. When the reagent interacts with a nucleobase, a detectable signal is produced and used to identify the nucleobase of the nucleic acid. Advantageously, the apparatus of this disclosure is specific to identifying nucleic acids.
Claims13
An apparatus for analyzing a nucleic acid sequence in a sample, the apparatus comprising a chamber, wherein the chamber comprises:
- (a) a first and a second electrode that form a tunnel gap through which the nucleic acid sequence can pass;
- (b) a first reagent attached to the first electrode and a second reagent attached to the second electrode, wherein the first and the second reagent are capable of selectively interacting with a nucleobase of the nucleic acid sequence via π-π interactions,
- wherein a detectable signal is produced when the nucleobase interacts with the first and second reagent via the π-π interactions and the first and second reagent are each an aromatic compound selected from pyrene, benzene, anthracene, benzo[e]pyrene, 2-(phenylethynyl)pyrene, 2-phenyl pyrene, 3-nitro-1H-pyrrole and 5-nitro-1H-indole, wherein the aromatic compound is unsubstituted or substituted with a substituent selected from nitro, phenyl, (C1-C6)alkyl substituted with phenyl, (C2-C6)alkenyl substituted with phenyl or (C2-C6)alkynyl substituted with phenyl or wherein the aromatic compound is a pyrene substituted with (C1-C6)mercaptoalkyl.
Dependencies are inferred from the claim text.