# US 11,118,231 B2 · miRNAs as biomarkers for distinguishing benign from malignant thyroid neoplasms

> Agents: this data is available as JSON from the patentgrep API and MCP server — see https://patentgrep.com/llms.txt

- Number: US 11,118,231 B2
- Document ID: US11118231B2
- Status: Granted
- Kind: B2
- Application number: 16/188,769
- Filed: 2018-11-13
- Published: 2019-10-10
- Granted: 2021-09-14
- Earliest claimed: 2010-11-17
- Assignees: Interpace Diagnostics Corporation
- Applicants: Interpace Diagnostics, LLC
- Inventors: Sylvie Beaudenon-Huibregtse; Ashish Choudhary
- Web page: https://patentgrep.com/patent/US11118231B2

## CPC

- C12Q 1/6886: for cancer (immunoassay for cancer G01N33/575)
- C12Q 2600/112: Disease subtyping, staging or classification
- C12Q 2600/158: Expression markers
- C12Q 2600/178: miRNA, siRNA or ncRNA
- C12Q: MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS (immunoassay G01N33/53); COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES

## Abstract

The present invention concerns methods and compositions for identifying a miRNA profile for a particular condition, such as thyroid nodules or thyroid cancer, and using the profile in the diagnosis of a patient for a condition, such as thyroid nodules or thyroid cancer.

## Claims

### Claim 1

A method of producing labeled nucleotide sequences from a thyroid sample from a subject, the method comprising
contacting the thyroid sample from the subject with labeled primers specific for a group of microRNAs consisting of miR-375, miR-146b-5p, mir-138-1*, and miR-204; and
reacting the sample with the labeled primers under conditions to amplify the microRNAs using polymerase chain reaction to produce the labeled nucleotide sequences.

### Claim 2 (depends on 1)

The method of claim 1, wherein the label is non-radioactive.

### Claim 3 (depends on 1)

The method of claim 1, wherein the label is radioactive.

### Claim 4 (depends on 1)

The method of claim 1, wherein the label is a UV spectrum emitting label.

### Claim 5 (depends on 1)

The method of claim 1, wherein the label is a fluorescent label.

### Claim 6 (depends on 1)

The method of claim 1, wherein the label is an enzymatic label.