Method for purifying and obtaining 3,6-anhydro-L-galactose using microorganisms

A method for purifying and obtaining 3,6-anhydro-L-galactose using microorganisms, comprising:

• reacting agarose or agar with a thermostable agarase, an exo-type agarase, and an α-neoagarobiose hydrolase, sequentially without pretreatment of the agarose or agar;
• culturing a microorganism having an ability to metabolize galactose in a cell culture media comprising a product resulting from the reacting the agarose or agar with the thermostable agarase, the exo-type agarase, and the α-neoagarobiose hydrolase;
• and
• collecting 3,6-anhydro-L-galactose by centrifugating or filtering a culture of the microorganism,
• wherein the thermostable agarase comprises amino acid sequences of SEQ ID NO:
• 1, the exo-type agarase comprises amino acid sequences of SEQ ID NO:
• 3, and the α-neoagarobiose hydrolase comprises amino acid sequences of SEQ ID NO:
• 4,
• wherein the microorganism having an ability to metabolize galactose is selected from Zymomonas mobilis and Pichia pastoris, and
• wherein the reacting the agarose or agar with the thermostable agarase is performed at a temperature ranging from 40 to 60° C. under a condition of 0 to 300 rpm and a pH ranging from 5 to 9 for 30 minutes to 7 days.