Method for preparing amines from aldehydes and ketones by biocatalysis
AssigneesCommissariat A L'energie Atomique Et Aux Energies Alternatives, Universite D'Evry Val D'Essonne (Ueve)
InventorsCarine Vergne-Vaxelaire, Véronique De Berardinis, Anne Zaparucha
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Abstract
The present invention relates to the preparation of amines from aldehydes and ketones by reductive amination with enzymes having a reductive aminase activity on aldehydes and ketones devoid of any carboxyl group gamma of the carbonyl group. The invention also relates to the enzymes per se and their uses in biocatalysis. The enzymes are derived from Mycobacterium smegmatis and vaccae, Cystobacter fuscus, Microbacterium sp. and Aminomonas paucivorans.
Claims18
A method for the reductive amination of a carbonyl-containing compound selected from aldehydes and ketones devoid of any carboxyl group at position gamma of the carbonyl group comprising contacting a carbonyl-containing compound selected from aldehydes and ketones devoid of any carboxyl group at position gamma of the carbonyl group with an enzyme having reductive aminase (RedAm) activity and comprising an amino acid sequence having at least 90% sequence identity with the amino acid sequence of SEQ ID NO:3, as a catalyst, to reductively aminate said carbonyl-containing compound to an amine-containing compound.
Dependencies are inferred from the claim text.