Enzyme-mediated depletion of adenosine and/or methylthioadenosine
AssigneeBoard of Regents, The University of Texas System
InventorsEverett Stone, Donjeta Gjuka
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Abstract
Methods and compositions related to the engineering of a protein with MTA/ADO-degrading enzyme activity are described. For example, in certain aspects there may be disclosed an MTase capable of degrading MTA/ADO. Furthermore, certain aspects of the invention provide compositions and methods for the treatment of cancer or SCID with an MTase using the disclosed proteins or nucleic acids.
Claims15
A composition for treating a methylthioadenosine phosphorylase-deficient cancer comprising:
- an enzyme capable of phosphorolysis of methylthioadenosine into methylthioribose-phosphate and adenine coupled to
- a compound capable of increasing a serum half-life of the enzyme;
- wherein the enzyme comprises a sequence having at least 90% identity to SEQ ID NO:
- 1, and
- wherein the compound capable of increasing a serum half-life of the enzyme is a polymer that increases a hydrodynamic radius of the enzyme.
A method of treating a patient having a tumor comprising administering to the patient an effective amount of a methylthioadenosine phosphorylase comprising a sequence having at least 90% identity to SEQ ID NO:
- 1.
A method of treating a patient having a tumor comprising:
- a) determining that the tumor comprises a homologous genetic deletion at chromosome 9p21 of a methylthioadenosine phosphorylase gene;
- and
- b) administering an enzyme capable of phosphorolysis of methylthioadenosine into methylthioribose-phosphate and adenine,
- wherein the enzyme comprises a sequence having at least 90% identity to SEQ ID NO:
- 1.
Dependencies are inferred from the claim text.