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US 11,117,921 B2Granted
Methods for synthesizing nucleotide analogues with disulfide linkers
AssigneeIsoPlexis Corporation
InventorsMong Sano Marma, Jerzy Olejnik, Ilia Korboukh
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Abstract
The present invention provides methods, compositions, mixtures and kits utilizing deoxynucleoside triphosphates comprising a 3′-O position capped by a group comprising methylenedisulfide as a cleavable protecting group and a detectable label reversibly connected to the nucleobase of said deoxynucleoside. Such compounds provide new possibilities for future sequencing technologies, including but not limited to Sequencing by Synthesis.
Claims8
A method, comprising:
- a) providing a 5′-O-(tert-butyldimethvisilyl)-iodo-2′-deoxynucleoside;
- b) treating said 5′-O-(tert-(butyldimethylsilyl)-iodo-2′-deoxynucleoside so as to produce a 5′-O-(tert-butyldimethylsilyl)-N-trifluoroacetyl-propargylamine-2′-deoxynucleoside intermediate;
- c) treating said 5′-O-(tert-butyldimethylsilyl)-N-trifluoroacetyl-propargylamine-2′-deoxynucleoside intermediate so as to produce a 5′-O-(tert-butyldimethylsilyl)-3′-O-(methyl-thio-2,4,6-trimethoxyphenyl)-N-trifluoroacetyl-propargylamine-2′-deoxynucleoside intermediate.
Dependencies are inferred from the claim text.