SARS-CoV-2 surrogate virus neutralization assay test kit

A kit for detecting neutralizing antibodies to a severe acute respiratory syndrome-related coronavirus (SARSr-COV), comprising:

• (i) a SARS-COV-1 or -2 spike protein or an ACE2-binding fragment of a SARS-COV-1 or -2 spike protein,
• (ii) a human ACE2 protein or a SARS-CoV-1 or -2 receptor binding domain (RBD) binding fragment of a human ACE2 protein thereof which binds specifically to the spike protein or the fragment thereof of (i), and
• a detection entity comprising a horseradish peroxidase, for detecting interaction between the spike protein or the ACE2-binding fragment of (i) and the human ACE2 protein or RBD binding fragment of (ii),
• wherein the spike protein or the ACE2-binding fragment of (i) is conjugated to the detection entity;
• and the human ACE2 protein or the RBD binding fragment of (ii) is immobilised on a solid support.