# US 11,112,404 B2 · Method for obtaining information of test substance > Agents: this data is available as JSON from the patentgrep API and MCP server — see https://patentgrep.com/llms.txt - Number: US 11,112,404 B2 - Document ID: US11112404B2 - Status: Granted - Kind: B2 - Application number: 15/951,375 - Filed: 2018-04-12 - Published: 2018-10-18 - Granted: 2021-09-07 - Earliest claimed: 2017-04-13 - Assignees: Sysmex Corporation - Applicants: Sysmex Corporation - Inventors: Kenji Akama; Toshihiro Watanabe; Masaya Okada; Kazuto Yamashita; Akshay Ganguly; Shigeki Iwanaga - Web page: https://patentgrep.com/patent/US11112404B2 ## CPC - G01N 33/54326: {Magnetic particles} - C07K 16/18: against material from animals or humans - G01N 33/533: with fluorescent label - G01N 33/54353: {with ligand attached to the carrier via a chemical coupling agent (coatings G01N33/54393)} - G01N 33/5436: {with ligand physically entrapped within the solid phase (liposomes G01N33/5432; immunological test elements G01N33/54386)} - G01N 33/54366: {Apparatus specially adapted for solid-phase testing} - G01N 33/582: {with fluorescent label} - G01N 33/6896: {Neurological disorders, e.g. Alzheimer's disease} - C07K 2317/70: characterized by effect upon binding to a cell or to an antigen - C07K 2317/92: Affinity (KD), association rate (Ka), dissociation rate (Kd) or EC50 value - C07K 2317/94: Stability, e.g. half-life, pH, temperature or enzyme-resistance - G01N 2333/4709: Amyloid plaque core protein - G01N: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES (measuring or testing apparatus or processes other than immunoassay, involving enzymes or microorganisms C12M, C12Q) - C07K: PEPTIDES (peptides in foodstuffs A23; obtaining protein compositions for foodstuffs, working-up proteins for foodstuffs A23J; preparations for medicinal purposes A61K; peptides containing beta-lactam rings C07D; cyclic dipeptides not having in their molecule any other peptide link than those which form their ring, e.g. piperazine-2,5-diones, C07D; ergot alkaloids of the cyclic peptide type C07D519/02; macromolecular compounds having statistically distributed amino acid units in their molecules, i.e. when the preparation does not provide for a specific; but for a random sequence of the amino acid units, homopolyamides and block copolyamides derived from amino acids C08G69/00; macromolecular products derived from proteins C08H1/00; preparation of glue or gelatine C09H; single cell proteins, enzymes C12N; genetic engineering processes for obtaining peptides C12N15/00; compositions for measuring or testing processes involving enzymes C12Q; investigation or analysis of biological material G01N33/00) ## Abstract Disclosed is a method for obtaining information of a test substance, the method including: forming a complex by causing a capture substance to bind to a test substance in a specimen; selectively collecting at least the complex from the specimen; immobilizing the complex collected from the specimen, onto a base plate; and obtaining information regarding a structure of the test substance from the complex immobilized on the base plate. ## Claims ### Claim 1 A method for obtaining information of a test substance, the method comprising: forming a complex by causing a capture substance to bind to a test substance in a specimen; selectively collecting at least the complex from the specimen; immobilizing the complex collected from the specimen, onto a base plate; and obtaining information regarding a structure of the test substance from the complex immobilized on the base plate. ### Claim 2 (depends on 1) The method for obtaining the information of the test substance of claim 1, wherein in the obtaining of the information regarding the structure of the test substance, at least one of size, morphology, structure, and aggregation degree of the test substance is obtained. ### Claim 3 (depends on 1) The method for obtaining the information of the test substance of claim 1, wherein the capture substance includes a capture substance for labeling the test substance with fluorescence. ### Claim 4 (depends on 1) The method for obtaining the information of the test substance of claim 1, wherein the capture substance includes a capture substance capable of binding to a solid phase. ### Claim 5 (depends on 1) The method for obtaining the information of the test substance of claim 1, wherein the capture substance includes a capture substance capable of binding to the base plate. ### Claim 6 (depends on 3) The method for obtaining the information of the test substance of claim 3, wherein the capture substance for labeling the test substance with fluorescence includes an antibody labeled with a fluorescent dye; and in the forming of the complex, the antibody labeled with the fluorescent dye is caused to bind to the test substance. ### Claim 7 (depends on 4) The method for obtaining the information of the test substance of claim 4, wherein after the solid phase is caused to bind to the complex through the capture substance capable of binding to the solid phase, the solid phase is selectively separated in the collecting of the complex from the specimen. ### Claim 8 (depends on 7) The method for obtaining the information of the test substance of claim 7, wherein in the collecting of the complex from the specimen, the complex is caused to be detached from the solid phase after the solid phase is selectively separated. ### Claim 9 (depends on 7) The method for obtaining the information of the test substance of claim 7, wherein the solid phase includes a magnetic particle, and the solid phase is selectively separated by attracting the magnetic particle by magnetic force. ### Claim 10 (depends on 7) The method for obtaining the information of the test substance of claim 7, wherein the capture substance capable of binding to the solid phase includes an antibody which binds to the test substance and a second binding substance which binds to the solid phase, the solid phase includes a second binding partner which specifically binds to the second binding substance, and binding between the complex and the solid phase is realized by binding between the test substance and the antibody of the capture substance capable of binding to the solid phase and by specific binding between the second binding substance and the second binding partner. ### Claim 11 (depends on 1) The method for obtaining the information of the test substance of claim 1, wherein the capture substance includes a capture substance capable of binding to the base plate and a capture substance capable of binding to a solid phase, and the capture substance capable of binding to the base plate and the capture substance capable of binding to the solid phase are different from each other. ### Claim 12 (depends on 1) The method for obtaining the information of the test substance of claim 1, wherein in the collecting of the complex from the specimen, the complex is separated from an impurity on the basis of at least one of a difference in specific gravity, a difference in size, a difference in electrical property, and a difference in immunoreaction between the complex and the impurity. ### Claim 13 (depends on 5) The method for obtaining the information of the test substance of claim 5, wherein the capture substance capable of binding to the base plate includes an antibody which binds to the test substance and a binding substance which binds to the base plate, the base plate includes a binding partner which specifically binds to the binding substance, and after the antibody included in the capture substance capable of binding to the base plate is caused to bind to the test substance, the complex is immobilized on the base plate through specific binding between the binding partner and the binding substance, in the immobilizing of the complex onto the base plate. ### Claim 14 (depends on 1) The method for obtaining the information of the test substance of claim 1, wherein after the collecting of the complex from the specimen, a capture substance capable of binding to the base plate is caused to bind to the test substance. ### Claim 15 (depends on 1) The method for obtaining the information of the test substance of claim 1, wherein in the obtaining of the information regarding the structure of the test substance, an image of the test substance is obtained by performing image capturing of the test substance on the base plate. ### Claim 16 (depends on 1) The method for obtaining the information of the test substance of claim 1, wherein in the collecting of the complex from the specimen, the test substance and an impurity are separated from each other, and the test substance is a protein as a test target contained in the specimen and the impurity includes a protein other than the protein as the test target. ### Claim 17 (depends on 1) The method for obtaining the information of the test substance of claim 1, wherein the test substance is amyloid β. ### Claim 18 (depends on 1) The method for obtaining the information of the test substance of claim 1, wherein the obtaining of the information regarding the structure of the test substance is performed by means of a microscope having a resolution exceeding a diffraction limit of light. ### Claim 19 A method for obtaining information of a test substance, the method comprising: causing a magnetic particle to bind to a test substance in a specimen; selectively collecting at least the test substance from the specimen by use of the magnetic particle bound to the test substance; immobilizing the test substance collected from the specimen, onto a base plate; and obtaining information regarding a structure of the test substance from the test substance immobilized on the base plate. ### Claim 20 A method for obtaining information of a test substance, the method comprising: forming a complex by causing a capture substance including a fluorescent dye to bind to a test substance in a specimen; selectively collecting at least the complex from the specimen; immobilizing the complex collected from the specimen, onto a base plate; and obtaining information regarding a structure of the test substance on the basis of bright spots corresponding to fluorescence emitted from a plurality of fluorescent dyes each bound to the complex immobilized on the base plate.