# US 11,112,400 B2 · Blood characteristic measurement > Agents: this data is available as JSON from the patentgrep API and MCP server — see https://patentgrep.com/llms.txt - Number: US 11,112,400 B2 - Document ID: US11112400B2 - Status: Granted - Kind: B2 - Application number: 15/749,116 - Filed: 2016-03-07 - Published: 2018-08-09 - Granted: 2021-09-07 - Earliest claimed: 2016-01-16 - Assignees: Hewlett-Packard Development Company, L.P. - Applicants: Hewlett-Packard Development Company, L.P. - Inventors: Shameed Sait M A; Rachel M. White; Chantelle Domingue; Manish Giri - Web page: https://patentgrep.com/patent/US11112400B2 ## CPC - G01N 33/4915: {using flow cells (flow cytometry G01N15/14)} - C12Q 1/56: involving blood clotting factors, e.g. involving thrombin, thromboplastin, fibrinogen - G01N 27/02: by investigating impedance - G01N 33/49: Blood {(chemical methods for determining blood cell populations G01N33/5094; chemical analysis of blood groups or blood types G01N33/80)} - G01N 33/561: Immunoelectrophoresis - G01N 33/86: involving blood coagulating time {or factors, or their receptors} - G01N: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES (measuring or testing apparatus or processes other than immunoassay, involving enzymes or microorganisms C12M, C12Q) - C12Q: MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS (immunoassay G01N33/53); COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES ## Abstract Examples herein provide a method. The method includes applying an electrical potential difference over a blood sample in a testing cassette of a microfluidic device, the cassette including a microfluidic channel through which the blood sample flows. The method includes measuring, over a duration of time, an electrical signal passing through the blood sample as the blood sample flows from a first end and coagulates at a second end of the microfluidic channel to obtain a measurement function as a function of time. The method also includes correlating the measurement function to a characteristic of the blood sample. ## Claims ### Claim 1 A method, comprising: applying an electrical potential difference over a blood sample in a testing cassette of a microfluidic device, the cassette comprising a microfluidic channel through which the blood sample flows, wherein the microfluidic channel comprises an array of at least three sensor areas, each of the at least three sensor areas comprising at least three electrodes located parallel to each other and perpendicular to a direction of flow in the microfluidic channel, wherein each sensor area comprises multiple electrodes; beginning as soon as the blood sample enters an input port of the testing cassette at a first end of the microfluidic channel: measuring an electrical signal passing through the blood sample as the blood sample flows from the first end to obtain a pre-coagulation property of a measurement function; and measuring an electrical signal passing through the blood sample as the blood sample coagulates at a second end of the microfluidic channel to obtain a coagulation property of the measurement function as a function of time through the multiple electrodes of the sensor areas; and correlating the measurement function to a characteristic of the blood sample. ### Claim 2 (depends on 1) The method of claim 1, wherein the electrical signal is at least one of voltage and impedance. ### Claim 3 (depends on 1) The method of claim 1, further comprising determining a property of the measurement function and correlating the determined property to the characteristic. ### Claim 4 (depends on 1) The method of claim 1, further comprising outputting the correlated measurement function on a mobile device. ### Claim 5 (depends on 1) The method of claim 1, further comprising storing at least one of the measurements and the correlated measurement function in a storage device. ### Claim 6 (depends on 1) The method of claim 1, further comprising comparing the correlated measurement function with a previously obtained and stored correlated measurement function. ### Claim 7 (depends on 1) The method of claim 1, further comprising comparing the correlated measurement function with a previously obtained correlated measurement function for determining a condition of a subject from which the blood sample is obtained based on the correlated measurement function. ### Claim 8 (depends on 1) The method of claim 1, wherein the characteristic includes at least one of: at least one of a flow rate and flow volume of red blood cells; arrival of platelets; at least one of activation, adhesion, aggregation, reaction, and plug formation of platelets; conversion of prothrombin to thrombin and fibrinogen to fibrin; and formation of a stable fibrin polymer. ### Claim 9 (depends on 1) The method of claim 1, wherein the characteristic is hematocrit, health, size, or shape of red blood cells, or combinations thereof. ### Claim 10 (depends on 1) The method of claim 1, further comprising operating a first electrode located at the input port and a second electrode located at the second end of the microfluidic channel, opposite from the input port, to begin measuring the electrical signal as soon as the blood sample enters the input port. ### Claim 11 (depends on 1) The method of claim 1, wherein the characteristic comprises flow rate of red blood cells. ### Claim 12 (depends on 1) The method of claim 1, wherein the characteristic comprises arrival or activation of platelets. ### Claim 13 A method, comprising: introducing a blood sample through an input port of a testing cassette; applying an electrical potential difference over the blood sample in the testing cassette, the cassette comprising a microfluidic channel through which the blood sample flows from the input port, wherein the microfluidic channel comprises an array of at least three sensor areas, each of the at least three sensor areas comprising at least three electrodes located parallel to each other and perpendicular to a direction of flow in the microfluidic channel, wherein each sensor area comprises multiple electrodes; beginning as soon as the blood sample enters the input port of the testing cassette at a first end of the microfluidic channel: measuring an electrical signal passing through the blood sample as the blood sample flows from the first end of the microfluidic channel to obtain a pre-coagulation property of a measurement function; and measuring an electrical signal passing through the blood sample as the blood sample coagulates at a second end of the microfluidic channel to obtain a coagulation property of the measurement function as a function of time through the electrodes of the sensor areas; determining a first property and second property of the measurement function before and during coagulation, respectively; and correlating at least one of the first property and second property to a characteristic of the blood sample. ### Claim 14 (depends on 13) The method of claim 13, further comprising comparing the correlated measurement function with a previously obtained correlated measurement function for determining a condition of a subject from which the blood sample is obtained based on the correlated measurement function. ### Claim 15 (depends on 13) The method of claim 13, further comprising continuously outputting at least one of the measurement function and the determined first property and second property. ### Claim 16 (depends on 13) The method of claim 13, wherein both the first property and the second property are employed to correlate to the characteristic. ### Claim 17 (depends on 13) The method of claim 13, wherein the testing cassette is detachable from the microfluidic device. ### Claim 18 A microfluidic device for testing a blood sample, the device comprising: a cassette comprising: an input port to receive a blood sample; an electrode at the input port to detect introduction of the blood sample; a microfluidic channel through which the blood sample flows; an array of at least three sensor areas within the microfluidic channel, each of the at least three sensor areas comprising at least three electrodes located parallel to each other and perpendicular to a direction of flow in the microfluidic channel, wherein each sensor area comprises multiple electrodes; a pump to pump the blood sample through the microfluidic channel; a number of impedance sensors located in the microfluidic channel; and an electronic device interface to connect to a computing device; a processor; and a non-transitory machine-readable medium, in communication with the processor, having instructions stored thereon which, when executed by the processor, cause the device to: apply an electrical potential difference over the blood sample using the electrode; measure an electrical signal passing through the blood sample as the blood sample flows from a first end of the microfluidic channel to obtain a pre-coagulation property of a measurement function; and measure an electrical signal passing through the blood sample as the blood sample coagulates at a second end of the microfluidic channel to obtain a coagulation property of the measurement function as a function of time through the multiple electrodes of the sensor areas; and correlate the measurement function to a characteristic of the blood sample. ### Claim 19 (depends on 18) The microfluidic device of claim 18, wherein a first sensor area and a second sensor area are on opposite sides of the input port. ### Claim 20 (depends on 18) The microfluidic device of claim 18, wherein the electronic device interface is a universal serial bus (USB) interface.