# US 11,112,339 B2 · Airborne agent collectors, methods, systems and devices for monitoring airborne agents > Agents: this data is available as JSON from the patentgrep API and MCP server — see https://patentgrep.com/llms.txt - Number: US 11,112,339 B2 - Document ID: US11112339B2 - Status: Granted - Kind: B2 - Application number: 16/292,249 - Filed: 2019-03-04 - Published: 2019-10-10 - Granted: 2021-09-07 - Earliest claimed: 2014-07-22 - Assignees: Diomics Corporation - Applicants: Diomics Corporation - Inventors: Thomas J. Kindt; John F. Steel; Beverly Lynn Wolgast - Web page: https://patentgrep.com/patent/US11112339B2 ## CPC - G01N 1/2273: {Atmospheric sampling} - C12Q 1/06: Quantitative determination - C12Q 1/24: Methods of sampling, or inoculating or spreading a sample; Methods of physically isolating an intact microorganisms - C12Q 1/6888: for detection or identification of organisms - C12Q 1/689: for bacteria - G01N 1/2205: {with filters} - G01N 33/56911: {Bacteria} - G01N 33/56983: {Viruses} - G01N 2001/022: {sampling for security purposes, e.g. contraband, warfare agents} - G01N 2001/028: {Sampling from a surface, swabbing, vaporising} - G01N 2333/005: from viruses - G01N 2333/195: from bacteria - G01N 2469/00: Immunoassays for the detection of microorganisms - Y02A 50/30: Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change - G01N: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES (measuring or testing apparatus or processes other than immunoassay, involving enzymes or microorganisms C12M, C12Q) - C12Q: MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS (immunoassay G01N33/53); COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES - Y02A: TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE ## Abstract Air flow systems, devices and methods for monitoring airborne agents include airborne agent collectors. Airborne agent collectors for collecting and detecting the presence and/or identification of an airborne agent(s) include a soluble and hydrophilic polycaprolactone (PCL) that has been treated with a base having a pH greater than 8 and in some embodiments, also treated with a neutralizing agent for increasing hydrophilicity. Detection and identification of airborne agents captured by an airborne agent collector can be performed using any suitable analytical protocols. Such protocols include nucleic acid assays, protein assays, and bioassays. The airborne agent collectors can be used for the detection and identification of nucleic acid from cells or organisms of any type in fixed structures and in mobile, portable devices or machines. ## Claims ### Claim 1 A method comprising the steps of: treating a soluble and hydrophilic polycaprolactone with a base having a pH greater than 8 and a neutralizing agent to increase the hydrophilicity of the polycaprolactone; flowing air through an air flow system comprising at least one airborne agent collector for collecting an airborne agent in the air, the at least one airborne agent collector comprising the treated soluble and hydrophilic polycaprolactone; removing the at least one airborne agent collector from the air flow system; and subjecting the removed at least one airborne agent collector or a portion thereof to an analysis to determine if the airborne agent is present on or in the agent collector or identify the airborne agent, if present on or in the agent collector, and wherein the subjecting step comprises exposing at least a portion of the treated soluble and hydrophilic polycaprolactone to an airborne agent extraction reagent to solubilize the at least a portion of the treated soluble and hydrophilic polycaprolactone. ### Claim 2 (depends on 1) The method of claim 1, wherein the airborne agent is an organism and a nucleic acid from the organism is collected by the at least one airborne agent collector, and wherein the subjecting step comprises contacting the at least one airborne agent collector or a portion thereof with at least one nucleic acid extraction reagent under conditions such that the soluble and hydrophilic polycaprolactone is solubilized or dissolved, resulting in a preparation comprising the nucleic acid separated from the at least one airborne agent collector or a portion thereof; and extracting the nucleic acid from the preparation. ### Claim 3 (depends on 2) The method of claim 2, further comprising analyzing the extracted nucleic acid. ### Claim 4 (depends on 2) The method of claim 2, wherein about 50% to about 95% of the nucleic acid from the organism is extracted. ### Claim 5 (depends on 2) The method of claim 2, wherein the nucleic acid is RNA, genomic DNA, cDNA, or mitochondrial DNA. ### Claim 6 (depends on 1) The method of claim 1, wherein the analysis is a PCR-based assay. ### Claim 7 (depends on 1) The method of claim 1, wherein the airborne agent is a virus selected from the group consisting of: Variola, Rift Valley Fever, Machupo, Ebola, Yellow Fever, Marburg, Hantaan, Dengue Fever, Lassa Fever, Encephalitis and Hemorrhagic Fever viruses, Influenza, Respiratory syncytial virus (RSV), pathogenic corona viruses, and genetically engineered or genetically modified virus. ### Claim 8 (depends on 1) The method of claim 1, further comprising replacing the removed at least one airborne agent collector with at least one replacement airborne agent collector. ### Claim 9 (depends on 1) The method of claim 1, wherein the air flow system is is installed in a drone. ### Claim 10 (depends on 1) The method of claim 1, wherein the air flow system is disposed within a free-standing and portable device. ### Claim 11 (depends on 1) The method of claim 1, wherein the airborne agent is an organism and a nucleic acid from the organism is collected by the airborne agent collector, and the step of subjecting the at least one airborne agent collector or a portion thereof to an analysis comprises generating a preparation comprising the nucleic acid, and analyzing the nucleic acid from the preparation. ### Claim 12 (depends on 11) The method of claim 11, wherein the airborne agent is a virus selected from the group consisting of: Variola, Rift Valley Fever, Machupo, Ebola, Yellow Fever, Marburg, Hantaan, Dengue Fever, Lassa Fever, Encephalitis and Hemorrhagic Fever viruses, Influenza, Respiratory syncytial virus (RSV), pathogenic corona viruses, and genetically engineered or genetically modified virus. ### Claim 13 (depends on 1) The method of claim 1, wherein the air flow system is an air filtration system comprising the at least one airborne agent collector and at least one filter for filtering particulate matter, and the airborne agent collector traps particles larger than about 2 microns. ### Claim 14 (depends on 13) The method of claim 13, wherein the at least one filter for filtering particulate matter traps particles larger than about 5 microns. ### Claim 15 (depends on 13) The method of claim 13, wherein the at least one filter for filtering particulate matter is a membrane filter, an activated carbon filter, a fiberglass filter, or a high-efficiency particulate air (HEPA) filter. ### Claim 16 (depends on 1) The method of claim 1, wherein the at least one airborne agent collector is a plurality of airborne agent collectors attached to a support prior to removal. ### Claim 17 (depends on 1) The method of claim 1, wherein the at least one airborne agent collector is formed as a sheet.