# US 11,112,338 B2 · Dual-image based bioimaging devices and techniques

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- Number: US 11,112,338 B2
- Document ID: US11112338B2
- Status: Granted
- Kind: B2
- Application number: 16/400,581
- Filed: 2019-05-01
- Published: 2019-08-22
- Granted: 2021-09-07
- Earliest claimed: 2015-12-23
- Assignees: San Heh Pharmaceutical Corporation
- Applicants: Vor, Inc.
- Inventors: TsungFeng Wu; Yu-Chen Chen
- Web page: https://patentgrep.com/patent/US11112338B2

## CPC

- G01N 1/10: in the liquid or fluent state {(burettes, pipettes B01L3/02; sampling of ground water E02D1/06; metering by volume of fluids or fluent solid material G01F11/00, G01F13/00)}
- A61B 5/6898: {Portable consumer electronic devices, e.g. music players, telephones, tablet computers}
- B01L 9/527: {for microfluidic devices, e.g. used for lab-on-a-chip}
- G01N 21/31: Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry {(G01N21/72 takes precedence)}
- G01N 21/47: Scattering, i.e. diffuse reflection (G01N21/25, G01N21/41 take precedence {G01N21/55 takes precedence})
- G01N 21/64: Fluorescence; Phosphorescence
- H10F 39/18: Complementary metal-oxide-semiconductor [CMOS] image sensors; Photodiode array image sensors
- B01L 3/502715: {characterised by interfacing components, e.g. fluidic, electrical, optical or mechanical interfaces}
- B01L 3/502769: {characterised by multiphase flow arrangements}
- B01L 2300/0654: Lenses; Optical fibres
- G01N 21/05: Flow-through cuvettes (G01N21/09 takes precedence; handling fluid samples G01N1/10)
- G01N 2021/0346: {Capillary cells; Microcells}
- G01N 2201/0221: Portable; cableless; compact; hand-held
- G01N: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES (measuring or testing apparatus or processes other than immunoassay, involving enzymes or microorganisms C12M, C12Q)
- A61B: DIAGNOSIS; SURGERY; IDENTIFICATION (usefulness limited to only animals A61D)
- B01L: CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- H10F: INORGANIC SEMICONDUCTOR DEVICES SENSITIVE TO INFRARED RADIATION, LIGHT, ELECTROMAGNETIC RADIATION OF SHORTER WAVELENGTH OR CORPUSCULAR RADIATION

## Abstract

Disclosed are methods, systems, and devices for detecting biological analytes in a sample. The disclosed technology can be used to obtain readings of analyte concentration in a sample by imaging scattered light from an angled narrow beam illuminator. A fluid sample containing one or more biological, organic, and inorganic analytes including proteins, viruses, bacteria, phages, toxins, proteins, peptides, DNA, RNA, hormones, chemicals, drugs, and isotopes can be transferred to a microfluidic device having one or more channels with dimensions to generate capillary action for sample transport. The geometry of the microfluidic device may include a reservoir and sensing area, wherein an immunometric reaction can take place for the narrow beam scanning. The test particle may be coated with a specific binding member that is used to bind the binding pair member on an analyte in a sample. Test particles form the binding and the particle/analyte conjugate may be scanned.

## Claims

### Claim 1

A bioimaging method, comprising:
illuminating, by an incident light source that generates incident light, a target at a predetermined angle through one or more slits, wherein the one or more slits restrict the incident light to a narrow beam;
illuminating, by the incident light source, an auto fluorescent layer that functions as an auto fluorescent light source to generate auto fluorescent light; and
imaging, by an imager, a first portion of scattered light from the target, wherein the first portion of scattered light is the incident light scattered by the target, wherein the imager is positioned to capture the first portion of scattered light and positioned to capture a second portion of the auto fluorescent light passing past the target, wherein the target generates a shadow on the imager from auto fluorescent light being blocked by the target, and wherein the predetermined angle causes incident light not scattered by the target to be blocked from the imager.

### Claim 2 (depends on 1)

The bioimaging method of claim 1, wherein the target includes a microfluidic device including one or more channels for receiving a sample fluid that includes target particles, wherein each channel includes an imaging region in the sample fluid to generate scattered light from target particles, wherein the target particles are illuminated by the incident light.

### Claim 3 (depends on 1)

The bioimaging method of claim 1, wherein the imager is included in a smartphone.

### Claim 4 (depends on 1)

The bioimaging method of claim 1, wherein a horizontal distance between the one or more slits causes a predetermined width of the narrow beam.