# US 11,111,611 B2 · Electrospun PNIPAAm/PCL fiber mats for aligned cell sheets

> Agents: this data is available as JSON from the patentgrep API and MCP server — see https://patentgrep.com/llms.txt

- Number: US 11,111,611 B2
- Document ID: US11111611B2
- Status: Granted
- Kind: B2
- Application number: 16/023,147
- Filed: 2018-06-29
- Published: 2019-01-03
- Granted: 2021-09-07
- Earliest claimed: 2017-06-30
- Assignees: Board of Regents, The University of Texas System
- Applicants: Board of Regents, The University of Texas System
- Inventors: Janeta Zoldan; Alicia Allen
- Web page: https://patentgrep.com/patent/US11111611B2

## CPC

- D04H 1/4334: Polyamides
- C12N 5/0068: {General culture methods using substrates (for specific animal cell type C12N5/06)}
- C12N 5/0618: {Cells of the nervous system}
- C12N 5/0654: {Osteocytes, Osteoblasts, Odontocytes; Bones, Teeth}
- C12N 5/0655: {Chondrocytes; Cartilage}
- C12N 5/0657: {Cardiomyocytes; Heart cells}
- C12N 5/0658: {Skeletal muscle cells, e.g. myocytes, myotubes, myoblasts}
- D04H 1/43: Acrylonitrile series
- D04H 1/43828: {sheath-core}
- D04H 1/43835: {Mixed fibres, e.g. at least two chemically different fibres or fibre blends}
- D04H 1/43838: {Ultrafine fibres, e.g. microfibres}
- D04H 1/728: by electro-spinning {(electro-spinning methods and apparatus D01D5/0007)}
- C12N 2533/30: Synthetic polymers
- C12N 2539/10: Coating allowing for selective detachment of cells, e.g. thermoreactive coating
- D04H 13/002: {characterised by the disposition or nature of their elements}
- D04H: MAKING TEXTILE FABRICS, e.g. FROM FIBRES OR FILAMENTARY MATERIAL (weaving D03; knitting D04B; braiding D04C; net-making D04G; sewing D05B; tufting D05C; finishing non-woven fabrics D06); FABRICS MADE BY SUCH PROCESSES OR APPARATUS, e.g. FELTS, NON-WOVEN FABRICS; COTTON-WOOL; WADDING {; NON-WOVEN FABRICS FROM STAPLE FIBRES, FILAMENTS OR YARNS, BONDED WITH AT LEAST ONE WEB-LIKE MATERIAL DURING THEIR CONSOLIDATION}(non-woven fabrics having an intermediate or external layer of a different kind, e.g. of woven fabric, B32B)
- C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00)

## Abstract

The present invention provides compositions comprising aligned fibers of electrospun PNIPAAm and poly (ε-caprolactone) (PCL) (denoted PNIPAAm/PCL fibers). The PNIPAAm/PCL compositions enable enhanced growth and detachment of intact anisotropic cell sheets. The compositions do not require chemical modification or resource-intensive techniques, thus saving time and expense, and have the potential to generate tissue-specific, aligned cell sheets for transplant studies.

## Claims

### Claim 1

A fiber mat comprising poly(N-isopropylacrylamide) (PNIPAAm) and poly(caprolactone) (PCL), wherein PNIPAAm content is at least 90%.

### Claim 2 (depends on 1)

The fiber mat of claim 1, having fibers with a diameter between about 1 and 3 μm.

### Claim 3 (depends on 1)

The fiber mat of claim 1, having fibers formed from a PNIPAAm core and a PCL shell.

### Claim 4 (depends on 1)

The fiber mat of claim 1, having PNIPAAm fibers and PCL fibers.

### Claim 5 (depends on 1)

The fiber mat of claim 1, having fibers arranged substantially in parallel.