Medium for the specific detection of resistant microorganisms
AssigneeBiomerieux
InventorsSylvain Orenga, Céline Roger-Dalbert, John Perry, Vanessa Chanteperdrix, Gilles Zambardi, Nathalie Bal
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Abstract
A method for distinguishing among a first group of microorganisms belonging to a first taxon of Gram negative bacteria, the first group of bacteria exhibiting a mechanism of resistance to a treatment; a second group of microorganisms belonging to a second taxon of Gram negative bacteria, the second taxon of bacteria being different than said first taxon, and exhibiting a mechanism of resistance to a treatment identical to the mechanism of the first group; and a third group of Gram negative bacteria that is not resistant to the treatment.
Claims7
A method of detecting and distinguishing between any extended-spectrum β-lactamase-producing E. coli bacteria (ESBL E. coli bacteria) and any extended-spectrum β-lactamase-producing KESC bacteria (ESBL KESC bacteria) that are present on a culture medium, the method comprising:
- inoculating a culture medium with a biological sample, wherein the culture medium comprises:
- a first substrate for detecting beta-galactosidase activity;
- a second substrate for detecting beta-glucuronidase activity;
- a third substrate for detecting desaminase activity;
- and
- a combination of antimicrobials throughout the entire culture medium, the combination comprising a cephalosporinase inhibitor and a third-generation cephalosporin;
- and
- simultaneously detecting and distinguishing between any ESBL E. coli bacteria and ESBL KESC bacteria that are present on the culture medium.
A method of detecting and distinguishing between any extended-spectrum β-lactamase-producing E. coli bacteria (ESBL E. coli bacteria) and any extended-spectrum β-lactamase-producing KESC bacteria (ESBL KESC bacteria) that are present on a culture medium, the method comprising:
- inoculating a culture medium with a biological sample, wherein the culture medium comprises:
- a first substrate for detecting beta-galactosidase activity;
- a second substrate for detecting beta-glucosidase activity;
- and
- a combination of antimicrobials throughout the entire culture medium, the combination comprising a cephalosporinase inhibitor and a third-generation cephalosporin.
Dependencies are inferred from the claim text.