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US 11,111,513 B2Granted
Methods of removing one or more compounds from a lignocellulosic hydrolysate via gas stripping, and related systems
AssigneePOET Research, Inc.
InventorsDavid Charles Carlson, Benjamin M. Gerdes, Casey C. Jenks, Ryan J. Bouza, Cory James Sarks
Life of this patent
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Abstract
The present disclosure includes methods and systems for removing one or more fermentation inhibitors (e.g., furfural) from a hydrolysate composition (e.g., a whole broth hydrolysate) by gas stripping the one or more fermentation inhibitors from the hydrolysate composition.
Claims14
A method of removing at least a portion of furfural from a hydrolysate composition, wherein the method comprises:
- a) providing a whole broth hydrolysate composition, wherein the providing comprises:
- i) providing lignocellulosic biomass comprising hemicellulose, cellulose, and lignin;
- ii) contacting the lignocellulosic biomass with an aqueous composition to hydrolyze at least a portion of the hemicellulose into one or more oligosaccharides and/or one or more pentoses, and form a first whole broth hydrolysate composition comprising at least pentose, cellulose, lignin, and furfural;
- and
- iii) enzymatically hydrolyzing at least a portion of the cellulose in the first whole broth hydrolysate composition to form a second whole broth hydrolysate composition comprising at least pentose, hexose, lignin, and furfural, wherein the furfural is present in the second whole broth hydrolysate in a first concentration;
- b) separating a first portion of the second whole broth hydrolysate composition and a second portion of the second whole broth hydrolysate composition from the second whole broth hydrolysate composition;
- c) injecting a first volume of a gas into the first portion of the second whole broth hydrolysate composition to provide a treated, whole broth hydrolysate composition, wherein the treated, whole broth hydrolysate composition comprises furfural in a second concentration, wherein the second concentration is less than the first concentration;
- d) recovering a second volume of the gas from the treated, whole broth hydrolysate composition, wherein the second volume of the gas comprises furfural;
- e) adding a first cell mass of yeast to the treated, whole broth hydrolysate composition;
- f) propagating the first cell mass of yeast in the treated, whole broth hydrolysate composition to form a second cell mass of yeast;
- g) adding the treated, whole broth hydrolysate composition, including the second cell mass of yeast, to the second portion of the second whole broth hydrolysate composition;
- and
- h) after “g”, fermenting the treated, whole broth hydrolysate composition, including the second cell mass of yeast, and the second portion of the second whole broth hydrolysate composition.
10 more elements
Dependencies are inferred from the claim text.