Method for producing phytosphingosine or sphinganine
AssigneesAjinomoto Co., Inc., Evolva SA
InventorsMarkus Schwab, Maud Babau, David Fischer, Anaelle Hatsch, Sabina de Andrade Pereira Tavares, Curt Aime Friis Nielsen, Jens Klein, Corina Daniela Wirdnam
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Abstract
A method for producing an objective substance such as phytosphingosine and sphinganine using yeast is provided. An objective substance is produced by cultivating yeast having an ability to produce the objective substance and modified so that the expression and/or activities of proteins encoded by LCB4 and CKA2 genes are reduced in a culture medium, and collecting the objective substance from cells of the yeast and/or the culture medium.
Claims15
A method for producing an objective substance, the method comprising:
- cultivating yeast having an ability to produce the objective substance in a culture medium;
- and
- collecting the objective substance from cells of the yeast and/or the culture medium,
- wherein the yeast has been modified so that the expression and/or activities of proteins encoded by LCB4, CKA2, and ORM2 genes are reduced, and
- wherein the objective substance is selected from the group consisting of phytosphingosine (PHS) and sphinganine (DHS), and
- wherein the culture medium contains cyclodextrin.
Dependencies are inferred from the claim text.