# US 11,111,502 B2 · ToLCNDV resistant melon plants > Agents: this data is available as JSON from the patentgrep API and MCP server — see https://patentgrep.com/llms.txt - Number: US 11,111,502 B2 - Document ID: US11111502B2 - Status: Granted - Kind: B2 - Application number: 16/606,979 - Filed: 2018-04-19 - Published: 2020-02-06 - Granted: 2021-09-07 - Earliest claimed: 2017-04-21 - Assignees: Nunhems B.V. - Applicants: Nunhems B.V. - Inventors: Juan Antonio Lizarzaburu Chavez; Jeffrey Skoneczka; Daniel Bellon Dona - Web page: https://patentgrep.com/patent/US11111502B2 ## CPC - A01H 6/344: {Cucumis melo [melon]} - A01H 1/045: {using molecular markers} - A01H 5/08: Fruits - C12N 15/8283: {for virus resistance} - C12Q 1/6895: for plants, fungi or algae - C12Q 2600/13: Plant traits - A01H: NEW PLANTS OR {NON-TRANSGENIC} PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES - C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00) - C12Q: MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS (immunoassay G01N33/53); COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES ## Abstract The present invention relates to ToLCNDV resistant melon plants comprising one or two introgression fragments in their genome. ## Claims ### Claim 1 A cultivated melon plant or plant cell comprising an introgression fragment on chromosome 11 and on chromosome 12 of a ToLCNDV resistant donor plant, wherein the introgression fragment on chromosome 11 comprises a sequence of the ToLCNDV resistant donor melon plant in-between SNP_01 at nucleotide 101 of SEQ ID NO: 1 and SNP_04 at nucleotide 101 of SEQ ID NO: 4, and wherein the introgression fragment on chromosome 12 comprises a sequence of the ToLCNDV resistant donor melon plant in-between SNP_05 at nucleotide 101 of SEQ ID NO: 5 and SNP_07 at nucleotide 101 of SEQ ID NO: 7, and wherein the introgression fragments on chromosome 11 and 12 are obtainable from seeds deposited under accession number NCIMB 42625, wherein the introgression fragment on chromosome 11 comprises a Thymine at nucleotide 101 of SEQ ID NO: 3 and the introgression fragment on chromosome 12 comprises a Guanine at nucleotide 101 of SEQ ID NO: 6. ### Claim 2 (depends on 1) The cultivated melon plant or plant cell according to claim 1, comprising an introgression fragment on chromosome 11 and on chromosome 12 of a ToLCNDV resistant donor plant, wherein the introgression fragment on chromosome 11 comprises a Adenine at nucleotide 101 of SEQ ID NO: 1 and/or a Guanine at nucleotide 101 of SEQ ID NO: 2 and/or a Thymine at nucleotide 101 of SEQ ID NO: 4, and wherein the introgression fragment on chromosome 12 comprises a Cytosine at nucleotide 101 of SEQ ID NO: 5 and/or a Thymine at nucleotide 101 of SEQ ID NO: 7. ### Claim 3 (depends on 1) The cultivated melon plant or plant cell according to claim 1, wherein the introgression fragment on chromosome 11 is in homozygous form and wherein the introgression fragment on chromosome 12 is in homozygous form or in heterozygous form. ### Claim 4 (depends on 1) The cultivated melon plant or plant cell according to claim 1, wherein the introgression fragment on chromosome 11 and on chromosome 12 is from a wild melon or wild relative of melon, optionally from the same wild melon or wild relative of melon donor accession. ### Claim 5 (depends on 1) The cultivated melon plant or plant cell according to claim 1, wherein the introgression fragment on chromosome 11 comprises a recessive QTL which confers an average TolCNDV resistance score of at least 7 on a scale of 1 (dead plant) to 9 (no symptoms) when the QTL is in homozygous form and wherein the introgression fragment on chromosome 12 comprises a partially dominant QTL which confers an average TolCNDV resistance score of at least 5 when the QTL is in homozygous form or in heterozygous form. ### Claim 6 (depends on 1) The cultivated melon plant or plant cell according to claim 1, wherein the plant or plant cell is an inbred plant or plant cell or an F1 hybrid plant or plant cell. ### Claim 7 (depends on 1) A seed which grows into a plant according to claim 1. ### Claim 8 (depends on 1) A cultivated melon fruit or fruit part comprising plant cells according to claim 1. ### Claim 9 (depends on 1) A cultivated melon plant propagation material comprising a cultivated melon plant cell according to claim 1. ### Claim 10 (depends on 6) A method for producing F1 hybrid seed comprising crossing the inbred line according to claim 6 with another melon plant and collecting seeds obtained from the cross. ### Claim 11 A method for producing hybrid cultivated melon seeds comprising a) crossing a first ToLCNDV resistant inbred melon plant comprising two chromosomes 11 each having an introgression fragment on chromosome 11 of a ToLCNDV resistant donor plant, the introgression fragment comprising QTL11 and a Thymine for SNP_03 at nucleotide 101 of SEQ ID NO: 3, or the sequence of the donor plant in-between SNP_03 at nucleotide 101 of SEQ ID NO: 3 and SNP_04 at nucleotide 101 of SEQ ID NO: 4, or in-between SNP_02 at nucleotide 101 of SEQ ID NO: 2 and SNP_03 at nucleotide 101 of SEQ ID NO: 3; or the introgression fragment comprising QTL11 and the resistant donor genotype for one or more or all of SNP_01, SNP_02, SNP_03 and SNP_04, wherein the resistant donor genotype for SNP_01, SNP_02, SNP_03 and SNP_04 is an Adenine at nucleotide 101 of SEQ ID NO: 1, a Guanine at nucleotide 101 of SEQ ID NO: 2, a Thymine at nucleotide 101 of SEQ ID NO: 3 and a Thymine at nucleotide 101 of SEQ ID NO: 4, respectively with a second ToLCNDV resistant inbred melon plant comprising two chromosomes 11 each having an introgression fragment on chromosome 11 of a ToLCNDV resistant donor plant, the introgression fragment comprising QTL11 and a Thymine for SNP_03 at nucleotide 101 of SEQ ID NO:3, or the sequence of the donor plant in-between SNP_03 at nucleotide 101 of SEQ ID NO:3 and SNP_04 at nucleotide 101 of SEQ ID NO: 4, or in-between SNP_02 at nucleotide 101 of SEQ ID NO: 2 and SNP_03 at nucleotide 101 of SEQ ID NO: 3; or the introgression fragment comprising QTL11 and the resistant donor genotype for one or more or all of SNP_01, SNP_02, SNP_03 and SNP_04 wherein the resistant donor genotype for SNP_01, SNP_02, SNP_03 and SNP_04 is an Adenine at nucleotide 101 of SEQ ID NO: 1, a Guanine at nucleotide 101 of SEQ ID NO: 2, a Thymine at nucleotide 101 of SEQ ID NO: 3 and a Thymine at nucleotide 101 of SEQ ID NO: 4, respectively; and b) collecting seeds obtained from the cross of a). ### Claim 12 A method of screening plants or plant parts, or DNA derived therefrom, for the presence of a fragment on chromosome 11 and/or 12 conferring ToLCNDV resistance, comprising: i) screening the genomic DNA for the SNP genotype of one or more or all of SNP_01 at nucleotide 101 of SEQ ID NO: 1, SNP_02 at nucleotide 101 of SEQ ID NO: 2, SNP_03 at nucleotide 101 of SEQ ID NO: 3 and SNP_04 at nucleotide 101 of SEQ ID NO: 4 and/or for one or more of SNP_05 at nucleotide 101 of SEQ ID NO: 5, SNP_06 at nucleotide 101 of SEQ ID NO: 6 and SNP_07 at nucleotide 101 of SEQ ID NO: 7; and ii) optionally selecting a plant or plant part which comprise the resistant donor genotype of one or more or all of SNP_01, SNP_02, SNP_03 and SNP_04 and/or for one or more or all of SNP_05, SNP_06 and SNP_07, wherein the resistant donor genotype for SNP_01, SNP_02, SNP_03 and SNP_04 is an Adenine at nucleotide 101 of SEQ ID NO: 1, a Guanine at nucleotide 101 of SEQ ID NO: 2, a Thymine at nucleotide 101 of SEQ ID NO: 3 and a Thymine at nucleotide 101 of SEQ ID NO: 4 and the resistant donor genotype for SNP_05, SNP_06 and SNP_07 is a Cytosine at nucleotide 101 of SEQ ID NO: 5, a Guanine at nucleotide 101 of SEQ ID NO: 6 and a Thymine at nucleotide 101 of SEQ ID NO: 7. ### Claim 13 (depends on 12) The method of claim 12, further comprising identifying a plant having ToLCNDV resistance comprising selecting a plant or plant part which comprise the resistant donor genotype of one or more or all of SNP_01, SNP_02, SNP_03 and SNP_04 and/or for one or more or all of SNP_05, SNP_06 and SNP_07. ### Claim 14 (depends on 11) The method according to claim 11, wherein the first and the second inbred melon plant in step a) further comprise an introgression fragment from chromosome 12 of a ToLCNDV resistant donor plant, the introgression fragment comprising QTL12 and a Guanine at nucleotide 101 of SEQ ID NO: 6, or the sequence of the donor plant in-between SNP_05 at nucleotide 101 of SEQ ID NO: 5 and SNP_07 at nucleotide 101 of SEQ ID NO: 7, or in-between SNP_05 at nucleotide 101 of SEQ ID NO: 5 and SNP_06 at nucleotide 101 of SEQ ID NO: 6, or in-between SNP_06 at nucleotide 101 of SEQ ID NO: 6 and SNP_07 at nucleotide 101 of SEQ ID NO: 7; or the introgression fragment comprising QTL12 and the resistant donor genotype for one or more or all of SNP_05 and SNP_06 and SNP_07, wherein the resistant donor genotype for SNP_05, SNP_06 and SNP_07 is a Cytosine at nucleotide 101 of SEQ ID NO: 5, a Guanine at nucleotide 101 of SEQ ID NO: 6 and a Thymine at nucleotide 101 of SEQ ID NO: 7. ### Claim 15 (depends on 3) A method for producing melon fruits, comprising a) growing a ToLCNDV resistant melon plant according to claim 3, b) harvesting the fruits of the melon plants grown in a).