# US 11,111,499 B2 · Isolated polynucleotides and polypeptides and methods of using same for increasing plant yield, biomass, growth rate, vigor, oil content, abiotic stress tolerance of plants and nitrogen use efficiency

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- Number: US 11,111,499 B2
- Document ID: US11111499B2
- Status: Granted
- Kind: B2
- Application number: 16/234,643
- Filed: 2018-12-28
- Published: 2019-04-25
- Granted: 2021-09-07
- Earliest claimed: 2011-05-03
- Assignees: Evogene Ltd.
- Applicants: Evogene Ltd.
- Inventors: Noa Matarasso; Eyal Emmanuel; David Panik; Inbal Nurith Dangoor; Hagai Karchi
- Web page: https://patentgrep.com/patent/US11111499B2

## CPC

- C12N 15/8247: {involving modified lipid metabolism, e.g. seed oil composition}
- C07K 14/415: from plants
- C12N 15/8246: {Non-starch polysaccharides, e.g. cellulose, fructans, levans}
- C12N 15/8261: {with agronomic (input) traits, e.g. crop yield}
- C12N 15/8271: {for stress resistance, e.g. heavy metal resistance}
- Y02A 40/146: Genetically Modified [GMO] plants, e.g. transgenic plants
- C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00)
- C07K: PEPTIDES (peptides in foodstuffs A23; obtaining protein compositions for foodstuffs, working-up proteins for foodstuffs A23J; preparations for medicinal purposes A61K; peptides containing beta-lactam rings C07D; cyclic dipeptides not having in their molecule any other peptide link than those which form their ring, e.g. piperazine-2,5-diones, C07D; ergot alkaloids of the cyclic peptide type C07D519/02; macromolecular compounds having statistically distributed amino acid units in their molecules, i.e. when the preparation does not provide for a specific; but for a random sequence of the amino acid units, homopolyamides and block copolyamides derived from amino acids C08G69/00; macromolecular products derived from proteins C08H1/00; preparation of glue or gelatine C09H; single cell proteins, enzymes C12N; genetic engineering processes for obtaining peptides C12N15/00; compositions for measuring or testing processes involving enzymes C12Q; investigation or analysis of biological material G01N33/00)
- Y02A: TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE

## Abstract

Provided are isolated polynucleotides encoding a polypeptide at least 80% homologous to the amino acid sequence selected from the group consisting of SEQ ID NOs: 757, 456-756, 758-774, 8385-10836, and 10838-14462; and isolated polynucleotide comprising nucleic acid sequences at least 80% identical to SEQ ID NO: 377, 1-376, 378-455, and 775-8384. Also provided are nucleic acid constructs comprising same, isolated polypeptides encoded thereby, transgenic cells and transgenic plants comprising same and methods of using same for increasing yield, biomass, growth rate, vigor, oil content, fiber yield, fiber quality, abiotic stress tolerance, and/or nitrogen use efficiency of a plant.

## Claims

### Claim 1

A method of increasing leaf number, growth rate of plot coverage and/or growth rate of rosette diameter of a plant, comprising:
(a) over-expressing within the plant a polypeptide comprising an amino acid sequence at least 87% identical to SEQ ID NO: 638, wherein said over-expressing of said polypeptide is effected by transforming a cell of the plant with an exogenous polynucleotide comprising a nucleic acid sequence encoding said polypeptide, and
(b) selecting said plant over-expressing said polypeptide for an increased trait selected from the group consisting of: leaf number, growth rate of plot coverage and growth rate of rosette diameter as compared to a native plant of the same species under the same growth conditions,
thereby increasing the leaf number, growth rate of plot coverage and growth rate of rosette diameter of the plant.

### Claim 2 (depends on 1)

The method of claim 1, wherein said amino acid sequence is at least 90% identical to the amino acid sequence set forth by SEQ ID NO: 638.

### Claim 3 (depends on 1)

The method of claim 1, wherein said amino acid sequence is at least 95% identical to the amino acid sequence set forth by SEQ ID NO:638.

### Claim 4 (depends on 1)

The method of claim 1, wherein said amino acid sequence is selected from the group consisting of SEQ ID NOs: 638, 10583, 10584, 10585, and 10586.

### Claim 5 (depends on 1)

The method of claim 1, wherein said trait is leaf number.

### Claim 6 (depends on 1)

The method of claim 1, further comprising:
(a) isolating regenerable portion of said plant selected according to claim 1 so as to obtain isolated regenerable portion of said selected plants; and
(b) regenerating plants from said isolated regenerable portion of said selected plants, wherein plants without the said trait are not isolated and not regenerated.

### Claim 7 (depends on 1)

The method of claim 1, wherein said nucleic acid sequence is selected from the group consisting of SEQ ID NOs: 437, 183, and 3903-3908.

### Claim 8 (depends on 1)

The method of claim 1, wherein said selecting is for increased leaf number, increased growth rate of plot coverage and increased growth rate of rosette diameter as compared to a native plant of the same species under the same growth conditions.

### Claim 9 (depends on 1)

The method of claim 1, wherein said selecting is for increased leaf number and increased growth rate of rosette diameter as compared to a native plant of the same species under the same growth conditions.