# US 11,111,498 B2 · High fructan cereal plants

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- Number: US 11,111,498 B2
- Document ID: US11111498B2
- Status: Granted
- Kind: B2
- Application number: 15/671,970
- Filed: 2017-08-08
- Published: 2018-06-21
- Granted: 2021-09-07
- Earliest claimed: 2008-07-17
- Assignees: Commonwealth Scientific and Industrial Research Organisation
- Applicants: Colin Leslie Dow Jenkins; Bryan Charles Clarke; Zhongyi Li; Matthew Kennedy Morell
- Inventors: Colin Leslie Dow Jenkins; Bryan Charles Clarke; Zhongyi Li; Matthew Kennedy Morell
- Web page: https://patentgrep.com/patent/US11111498B2

## CPC

- C12N 15/8245: {involving modified carbohydrate or sugar alcohol metabolism, e.g. starch biosynthesis}
- A61P 1/00: Drugs for disorders of the alimentary tract or the digestive system
- A61P 3/00: Drugs for disorders of the metabolism (of the blood or the extracellular fluid A61P7/00)
- A61P 3/02: Nutrients, e.g. vitamins, minerals
- A61P 19/08: for bone diseases, e.g. rachitism, Paget's disease
- G01N 33/50: Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing (measuring or testing processes involving enzymes or microorganisms, compositions or test papers therefor; processes for forming such compositions, condition responsive control in microbiological or enzymological processes C12Q)
- G01N 2400/38: Heteroglycans, i.e. polysaccharides having more than one sugar residue in the main chain in either alternating or less regular sequence, e.g. gluco- or galactomannans, Konjac gum, Locust bean gum or Guar gum
- C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00)
- A61P: SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- G01N: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES (measuring or testing apparatus or processes other than immunoassay, involving enzymes or microorganisms C12M, C12Q)

## Abstract

The invention provides cereal plants having a high level of fructan useful for the production of a range of food, beverage, nutraceutical and pharmaceutical products. The invention provides methods of producing high-fructan products from plants modified to comprise a reduced level of an endogenous polypeptide with starch synthase activity, and products so produced. In some embodiments, plants are modified by introduction of an agent such as a nucleic acid molecule which down regulates endogenous starch synthase II gene expression.

## Claims

### Claim 1

A method of identifying a variety of wheat grain which has increased levels of fructan comprising:
(i) obtaining wheat grain of a mutant hexaploid wheat plant grown in a field,
wherein said wheat grain has a reduced level or activity of an endogenous starch synthase (SSII) polypeptide relative to grain from a wild-type wheat plant grown under the same environmental conditions as the mutant hexaploid wheat plant,
wherein said wheat grain is homozygous for null alleles of an SSII gene in each of the A, B, and D genomes, wherein the homozygous alleles of the SSII gene of one or more of the A, B, and/or D genome(s) comprises a single base-pair substitution, insertion, or deletion;
(ii) determining the amount of fructan in the wheat grain; and
(iii) if the wheat grain comprises at least 3% fructan as a percentage of the wheat grain weight, selecting the wheat grain.