# US 11,111,491 B2 · Methods for improving traits in plants

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- Number: US 11,111,491 B2
- Document ID: US11111491B2
- Status: Granted
- Kind: B2
- Application number: 16/496,445
- Filed: 2018-03-27
- Published: 2020-04-16
- Granted: 2021-09-07
- Earliest claimed: 2017-03-28
- Assignees: Plantarcbio Ltd.
- Applicants: Plantarcbio Ltd.
- Inventors: Dror Shalitin; Noam Grimberg; Arava Shatil Cohen
- Web page: https://patentgrep.com/patent/US11111491B2

## CPC

- C12N 15/1075: {by coupling phenotype to genotype, not provided for in other groups of this subclass}
- C12N 15/102: {Mutagenizing nucleic acids}
- C12N 15/1058: {Directional evolution of libraries, e.g. evolution of libraries is achieved by mutagenesis and screening or selection of mixed population of organisms}
- C12N 15/8273: {for drought, cold, salt resistance}
- Y02A 40/146: Genetically Modified [GMO] plants, e.g. transgenic plants
- C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00)
- Y02A: TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE

## Abstract

The present invention discloses a method for screening for and identifying a desirable plant improving trait, said method comprises steps of: (a) obtaining genetic material from a sampling of a predefined source and (b) constructing an expression library from said genetic material. The aforementioned method further comprises steps of: (c) producing plants transformed with said expression library at a transformation efficiency of at least 0.05%-30%, representing at least 102-1010 transgenes; (d) screening for transformed plants expressing said desirable trait; and (e) identifying said transgene of said transformed plants expressing said desirable trait.

## Claims

### Claim 1

A plant comprising a transgene
encoding a polypeptide sequences having at least 90% identity to the polypeptide sequences set forth in SEQ ID NO: 213 and wherein the plant has one or more of the following characteristics: improved drought resistance, increased biomass, increased salinity tolerance.

### Claim 2 (depends on 1)

The plant according to claim 1, wherein said plant has at least two plant improving traits as compared to a plant of the same species lacking said transgene.

### Claim 3 (depends on 1)

The plant of claim 1, wherein the transgene identified has a polynucleotide sequences having at least 80% identity to the polynucleotide sequences set forth in SEQ ID NO:56.

### Claim 4 (depends on 1)

The plant according to claim 1, wherein the plant is further characterized by improved nutrition value of the crop, improved grain yield, increased herbicide or chemical resistance or tolerance, increased resistance to cold, increased resistance to hear, improved fertilizer uptake, improved fertilizer usage or any combination thereof.