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US 11,111,482 B2Granted
Genetically modified lactate-consuming yeasts and fermentation processes using such genetically modified yeasts
AssigneeCargill, Incorporated
InventorsArlene M. Fosmer, Christopher K. Miller, Gregory Michael Poynter, Brian Jeffrey Rush, Jon Veldhouse
Life of this patent
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Abstract
The present invention relates to genetically modified yeasts that can use lactate as a carbon source to produce a fermentation product. In one aspect, the yeasts can consume gluconse and lactate simultaneously to produce ethanol. In one aspect, the genetically modified yeast is transformed to include a monocarboxylic/monocarboxylate transporter. In one aspect, the yeast can include one or more heterologous genes encoding lactate dehydrogenase (cytochrome) (EC 1.1.2.3 and/or 1.1.2.4).
Claims9
A genetically modified yeast comprising:
- a heterologous gene encoding monocarboxylic/monocarboxylate transporter polypeptide having monocarboxylic/monocarboxylate transporter activity, wherein the said monocarboxylic/monocarboxylate transporter polypeptide comprises an amino acid sequence having at least 90% sequence identity to any one of the following amino acid sequences:
- SEQ ID NO:
- 24, SEQ ID NO:
- 26, SEQ ID NO:
- 28, SEQ ID NO:
- 76, or SEQ ID NO:
- 78, and
- one or more heterologous genes encoding a lactate dehydrogenase polypeptide having lactate dehydrogenase activity, wherein the said lactate dehydrogenase polypeptide comprises an amino acid sequence having at least 90% sequence identity to any one of the following amino acid sequences:
- SEQ ID NO:
- 40, SEQ ID NO:
- 42, SEQ ID NO:
- 64, SEQ ID NO:
- 66, SEQ ID NO:
- 68, SEQ ID NO:
- 70, SEQ ID NO:
- 72, or SEQ ID NO:
- 74, and
- wherein the yeast is capable of consuming lactate and producing ethanol at a titer greater than 80 g/L when the yeast is present in a fermentation medium comprising lactate and hexose.
15 more elements
Dependencies are inferred from the claim text.