# US 11,111,479 B2 · Methods and kits for guided stem cell differentiation

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- Number: US 11,111,479 B2
- Document ID: US11111479B2
- Status: Granted
- Kind: B2
- Application number: 15/652,938
- Filed: 2017-07-18
- Published: 2019-01-24
- Granted: 2021-09-07
- Earliest claimed: 2017-07-18
- Applicants: Shengyuan Yang
- Inventors: Shengyuan Yang
- Web page: https://patentgrep.com/patent/US11111479B2

## CPC

- C12N 5/0653: {Adipocytes; Adipose tissue}
- C12N 5/0654: {Osteocytes, Osteoblasts, Odontocytes; Bones, Teeth}
- C12N 5/0663: {Bone marrow mesenchymal stem cells (BM-MSC)}
- C12N 2506/1384: from adipose-derived stem cells [ADSC], from adipose stromal stem cells
- C12N 2513/00: 3D culture
- C12N 2531/00: Microcarriers
- C12N 2533/12: Glass
- C12N 2535/00: Supports or coatings for cell culture characterised by topography
- C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00)

## Abstract

The present disclosure provides methods, and related kits, for directing cell attachment and spreading on a substrate and inducing isotropic spreading of cells; provides methods, and related kits, for cell sorting; and further provides methods, and related kits, for guided induction of stem cell differentiation.

## Claims

### Claim 1

A method to modulate the relative osteogenic gene expression of a mesenchymal stem cell in the presence of osteocyte differentiation induction media by culturing the mesenchymal stem cell on a ball having a diameter and choosing a diameter between 500 μm and 6 mm, excluding 3 mm, comprising: culturing the mesenchymal stem cell on a ball, comprising the chosen diameter between about 500 μm and about 6 mm excluding 3 mm, wherein the level of the relative osteogenic gene expression of the mesenchymal stem cell decreases as the diameter of the substrate ball increases from about 500 μm to about 3 mm, the level of the relative osteogenic gene expression of the mesenchymal stem cell increases as the diameter of the ball increases from about 3 mm to about 6 mm and the level of the relative osteogenic gene expression of the mesenchymal stem cell on a ball of a diameter about 3 mm is comparable to that of the mesenchymal stem cell on a flat plate.

### Claim 2 (depends on 1)

The method of claim 1, wherein the ball comprises a coating selected from the group consisting of a cell adhesive, a cell adhesion-promotor, a cell repellent, or a combination thereof.

### Claim 3 (depends on 1)

The method of claim 1, wherein the ball is a glass ball.