# US 11,111,469 B2 · Device for cell culture

> Agents: this data is available as JSON from the patentgrep API and MCP server — see https://patentgrep.com/llms.txt

- Number: US 11,111,469 B2
- Document ID: US11111469B2
- Status: Granted
- Kind: B2
- Application number: 16/302,157
- Filed: 2017-05-05
- Published: 2019-05-16
- Granted: 2021-09-07
- Earliest claimed: 2016-05-18
- Assignees: Politecnico Di Milano
- Applicants: Politecnico Di Milano
- Inventors: Manuela Teresa Raimondi; Matteo Lagana
- Web page: https://patentgrep.com/patent/US11111469B2

## CPC

- C12M 23/38: {Caps; Covers; Plugs; Pouring means}
- B01L 3/50853: {with covers or lids (closures for test tubes B01L3/50825)}
- C02F 1/008: {Control or steering systems not provided for elsewhere in subclass C02F}
- C02F 1/76: with halogens or compounds of halogens {(C02F1/4674 takes precedence)}
- C12M 23/12: {Well or multiwell plates (C12M25/04 takes precedence)}
- C12M 23/22: {Transparent or translucent parts (glassware for laboratory use B01L3/00)}
- C12M 23/46: {Means for fastening}
- C12M 29/10: {Perfusion}
- B01L 2300/0822: Slides
- B63J 1/00: Arrangements of installations for producing fresh water, e.g. by evaporation and condensation of sea water {(treatment of water in general C02F)}
- C02F 1/006: {Water distributors either inside a treatment tank or directing the water to several treatment tanks; Water treatment plants incorporating these distributors, with or without chemical or biological tanks (for settling tanks B01D21/24)}
- C02F 2103/02: Non-contaminated water, e.g. for industrial water supply
- C02F 2201/001: Build in apparatus for autonomous on board water supply and wastewater treatment (e.g. for aircrafts, cruiseships, oil drilling platforms, railway trains, space stations)
- C02F 2209/003: Downstream control, i.e. outlet monitoring, e.g. to check the treating agents, such as halogens or ozone, leaving the process
- C02F 2209/006: comprising a software program or a logic diagram
- C02F 2209/29: Chlorine compounds
- C02F 2209/44: Time
- C02F 2303/04: Disinfection
- C02F 2303/185: The treatment agent being halogen or a halogenated compound
- C02F 2307/14: Treatment of water in water supply networks, e.g. to prevent bacterial growth
- C12M: APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; {APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS}
- B01L: CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- C02F: TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE (separation in general B01D; special arrangements on waterborne vessels of installations for treating water, waste water or sewage, e.g. for producing fresh water, B63J; adding materials to water to prevent corrosion C23F; treating radioactively-contaminated liquids G21F9/04; regeneration of reactants for recirculation into processes, see the relevant places for the processes)
- B63J: AUXILIARIES ON VESSELS

## Abstract

A device for cell culture comprising: a main body (11); said main body (11) comprises a plurality of circular portions (21-23); said device comprises a plurality of caps (51-53); each of said plurality of caps (51-53) comprises a base structure (58) having a circular hole (57), housing an upper slide (54) and an elastomeric layer (56) secured to said slide (54); said elastomeric layer (56) has a rectangular hole (59); said plurality of caps (51-53) being adapted to cooperate with said plurality of circular portions (21-23); each of said plurality of circular portions (21-23) each comprise an inlet hole (64-66) and an outlet hole (67-69) aligned with the long side of said rectangular hole (59), to perfuse the culture chamber (12-14) located in said rectangular hole (59) of said elastomeric layer (56).

## Claims

### Claim 1

A device for cell culture comprising: a main body (11);
wherein: said main body (11) comprises a plurality of circular portions (21-23); said device comprises a plurality of caps (51-53); each of said plurality of caps (51-53) comprises a base structure (58) having a circular hole (57), housing an upper slide (54) and an elastomeric layer (56) secured to said slide (54); each elastomeric layer (56) having a rectangular hole (59); said plurality of caps (51-53) being adapted to cooperate with said plurality of circular portions (21-23); and each of said plurality of circular portions (21-23) each comprise an inlet hole (64-66) and an outlet hole (67-69) aligned with a long side of each rectangular hole (59), to perfuse a culture chamber (12-14) located in said rectangular hole (59) of each elastomeric layer (56).

### Claim 2 (depends on 1)

The device according to claim 1, characterized in that each rectangular hole (59) is a through hole.

### Claim 3 (depends on 1)

The device according to claim 1 characterized in that each of said plurality of caps (51-53) has a base structure (58) of elongated shape.

### Claim 4 (depends on 1)

The device according to claim 1, characterized in that said main body (11) is rectangular and comprises edges (15, 16); and in that on long sides of said main body (11), the edges (16) have a plurality of pairs of openings (17, 18, 19) at each culture chamber (12, 14).

### Claim 5 (depends on 1)

The device according to claim 1, characterized in that each of said plurality of circular portions (21-23) is surrounded by a circular recess (24-26) housing a circular magnet (27) in the shape of a washer; and each of said plurality of caps (51-53) comprises a circular magnet (55) in the shape of a washer.

### Claim 6 (depends on 1)

The device according to claim 1, characterized in that each of said plurality of caps (51-53) engages in pairs of openings (17-19) present in lateral edges (16) of said main body (11).

### Claim 7 (depends on 1)

The device according to claim 1, characterized in that each culture chamber is physically separated from an adjacent chamber.

### Claim 8 (depends on 1)

The device according to claim 1, characterized in that said elastomeric layer (56) of each cap opposes each of said plurality of circular portions (21-23).

### Claim 9 (depends on 1)

The device according to claim 1 characterized in that a rear of the main body (11) is provided with two pairs of clips (41-43) for each of said plurality of circular portions (21-23).

### Claim 10 (depends on 1)

A method for producing a device for cell culture according to claim 1 comprising:
forming the device using injection moulding.