# US 11,110,462 B2 · Systems and methods for a thermal cycler heated cover > Agents: this data is available as JSON from the patentgrep API and MCP server — see https://patentgrep.com/llms.txt - Number: US 11,110,462 B2 - Document ID: US11110462B2 - Status: Granted - Kind: B2 - Application number: 16/515,533 - Filed: 2019-07-18 - Published: 2020-01-16 - Granted: 2021-09-07 - Earliest claimed: 2015-12-22 - Assignees: Life Technologies Corporation - Applicants: Life Technologies Corporation - Inventors: Zeqi Tan; Wuh Ken Loh; Siew Yin Lee; Kuan Moon (Bernard) Boo - Web page: https://patentgrep.com/patent/US11110462B2 ## CPC - B01L 7/52: {with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples} - G01N 35/026: {having blocks or racks of reaction cells or cuvettes} - B01L 2200/025: Align devices or objects to ensure defined positions relative to each other - B01L 2200/143: Quality control, feedback systems - B01L 2300/041: Connecting closures to device or container - B01L 2300/045: whereby the whole cover is slidable - B01L 2300/046: Function or devices integrated in the closure - B01L 2300/0609: Holders integrated in container to position an object - B01L 2300/0663: Whole sensors - B01L 2300/18: Means for temperature control - B01L 2300/1827: using resistive heater - B01L: CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE - G01N: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES (measuring or testing apparatus or processes other than immunoassay, involving enzymes or microorganisms C12M, C12Q) ## Abstract A thermal cycler system for use with a sample holder configured to receive a plurality of samples includes a sample block configured to receive the sample holder, a cover lid configured to move in a direction toward the sample block from an open position to a closed position, a heated cover operatively coupled to the cover lid and configured to move in a direction toward the sample block from a raised position to a first lowered position, in which the heated cover contacts the sample holder when the sample holder is received by the sample block, and a drive assembly including a motion guide configured to move in a direction toward the sample block from a first position, wherein the cover lid is in the open position and the heated cover is in the raised position, to a second position, wherein the cover lid is in the closed position. ## Claims ### Claim 1 A thermal cycler system comprising: a sample block comprising a sample holder receiving surface configured to receive the sample holder; and a cover assembly moveable in a first direction parallel to the sample holder receiving surface between an open position and a closed position relative to the sample holder receiving surface, wherein the cover assembly covers the sample holder receiving surface in the closed position and exposes the sample holder receiving surface in the open position, wherein the cover assembly comprises: a platen moveable, in the closed position of the cover assembly, in a second direction perpendicular to the first direction between a raised position and a lowered position relative to the sample holder receiving surface, a cover lid over the platen, the cover lid comprising a cover surface and opposing side walls extending from the cover surface, an actuatable linkage coupled to and extending transversely across the platen in a direction perpendicular to the first direction, opposite ends of the actuatable linkage being received in recesses in an interior surface of the opposing side walls of the cover lid, the recesses oriented in the second direction, the opposite ends being movable along the recesses in the second direction to move the actuatable linkage in the second direction between a raised position and a lowered position, and an elastically deformable member between the actuatable linkage and the platen, the elastically deformable member arranged to exert a compressive force against the platen in the lowered position of the platen and the actuatable linkage. ### Claim 2 (depends on 1) The system of claim 1, wherein the platen is configured to be heated. ### Claim 3 (depends on 1) The system of claim 1, further comprising a drive assembly operably coupled to the cover assembly, the drive assembly configured to impart a drive force on the cover assembly in the first direction. ### Claim 4 (depends on 3) The system of claim 3, further comprising a motion guide coupled to the cover assembly and the drive assembly, the motion guide positioned to transfer the drive force from the drive assembly to the cover assembly, the motion guide and the cover assembly moving together over a first range of motion in the first direction in response to the drive force. ### Claim 5 (depends on 4) The system of claim 4, wherein: the cover lid further comprises a rear wall located at a trailing end of the cover lid in the first range of motion of the cover assembly from the open position to the closed position, and the motion guide comprises a crossbar parallel the rear wall. ### Claim 6 (depends on 1) The system of claim 1, wherein the actuatable linkage comprises a bar. ### Claim 7 (depends on 1) The system of claim 1, wherein the actuatable linkage moves in the second direction between the raised position and the lowered position when the cover assembly is in the closed position. ### Claim 8 (depends on 1) The system of claim 1, further comprising one or more sensors configured to detect one or both of the platen and the actuatable linkage being in the raised position and the lowered position. ### Claim 9 A thermal cycler system comprising: a sample block comprising a sample holder receiving surface configured to receive the sample holder; a cover assembly moveable in a first direction parallel to the sample holder receiving surface between an open position and a closed position relative to the sample holder receiving surface, wherein the cover assembly covers the sample holder receiving surface in the closed position and exposes the sample holder receiving surface in the open position, wherein the cover assembly comprises: a platen moveable, in the closed position of the cover assembly, in a second direction perpendicular to the first direction between a raised position and a lowered position relative to the sample holder receiving surface, a cover lid over the platen, the cover lid comprising a cover surface and opposing side walls extending from the cover surface, an actuatable linkage coupled to and extending transversely across the platen in a direction perpendicular to the first direction, opposite ends of the actuatable linkage being received in recesses in an interior surface of the opposing side walls of the cover lid, the opposite ends being movable along the recesses in the second direction to move the actuatable linkage in the second direction between a raised position and a lowered position, and an elastically deformable member between the actuatable linkage and the platen, the elastically deformable member arranged to exert a compressive force against the platen in the lowered position of the platen and the actuatable linkage; and a motion guide coupled to the cover assembly, the motion guide configured to transfer a drive force to the cover assembly, wherein in response to the drive force: the motion guide and the cover assembly are movable together over a first range of motion between the open position and the closed position of the cover assembly, and in the closed position, the motion guide is movable relative to the cover assembly. ### Claim 10 (depends on 9) The system of claim 9, further comprising a stop, the cover assembly abutting against the stop in the closed position to prevent further movement of the cover assembly in the first direction in response to continued drive force. ### Claim 11 (depends on 9) The system of claim 9, wherein the platen is moveable over a second range of motion in the second direction in response to movement of the motion guide relative to the cover assembly. ### Claim 12 (depends on 11) The system of claim 11, wherein the actuatable linkage moves in the second direction between the raised position and the lowered position during the second range of motion. ### Claim 13 A thermal cycler system comprising: a sample block comprising a sample holder receiving surface configured to receive the sample holder; a cover assembly moveable in a first direction parallel to the sample holder receiving surface between an open position and a closed position relative to the sample holder receiving surface, wherein the cover assembly covers the sample holder receiving surface in the closed position and exposes the sample holder receiving surface in the open position, wherein the cover assembly comprises: a platen moveable, in the closed position of the cover assembly, in a second direction perpendicular to the first direction between a raised position and a lowered position relative to the sample holder receiving surface, a cover lid over the platen, the cover lid comprising a cover surface and opposing side walls extending from the cover surface, an actuatable linkage coupled to and extending transversely across the platen in a direction perpendicular to the first direction, opposite ends of the actuatable linkage being received in recesses in an interior surface of the opposing side walls of the cover lid, the opposite ends being movable along the recesses in the second direction to move the actuatable linkage in the second direction between a raised position and a lowered position, and an elastically deformable member between the actuatable linkage and the platen, the elastically deformable member arranged to exert a compressive force against the platen in the lowered position of the platen and the actuatable linkage; and a motion guide coupled to the cover assembly, the motion guide configured to transfer a drive force to the cover assembly, wherein the actuatable linkage is coupled to the motion guide via a cam-slot mechanism. ### Claim 14 (depends on 13) The system of claim 13, the actuatable linkage comprising protrusions at the opposite ends of the actuatable linkage, the protrusions received in respective cam slots of the motion guide. ### Claim 15 (depends on 14) The system of claim 14, wherein the cam slots extend in a direction oblique to the first and second directions. ### Claim 16 (depends on 14) The system of claim 14, wherein the protrusions move in the respective cam slots in a direction oblique to the first and second directions during movement of the actuatable linkage between the raised position and the lowered position. ### Claim 17 A thermal cycler system comprising: a sample block comprising a sample holder receiving surface configured to receive the sample holder; a cover assembly moveable in a first direction parallel to the sample holder receiving surface between an open position and a closed position relative to the sample holder receiving surface, wherein the cover assembly covers the sample holder receiving surface in the closed position and exposes the sample holder receiving surface in the open position, wherein the cover assembly comprises: a platen moveable, in the closed position of the cover assembly, in a second direction perpendicular to the first direction between a raised position and a lowered position relative to the sample holder receiving surface, a cover lid over the platen, the cover lid comprising a cover surface, opposing side walls extending from the cover surface, and a rear wall located at a trailing end of the cover lid based on movement of the cover assembly from the open position to the closed position, an actuatable linkage coupled to and extending transversely across the platen in a direction perpendicular to the first direction, opposite ends of the actuatable linkage being received in recesses in an interior surface of the opposing side walls of the cover lid, the opposite ends being movable along the recesses in the second direction to move the actuatable linkage in the second direction between a raised position and a lowered position, and an elastically deformable member between the actuatable linkage and the platen, the elastically deformable member arranged to exert a compressive force against the platen in the lowered position of the platen and the actuatable linkage; and a motion guide coupled to the cover assembly, the motion guide comprises a crossbar parallel to the rear wall, wherein the elastically deformable member is a first elastically deformable and the system further comprises a second elastically deformable member, the second elastically deformable member coupled to the rear wall and the crossbar. ### Claim 18 (depends on 17) The system of claim 17, wherein the compressive force is a first compressive force, and the second elastically deformable member is configured to exert a second compressive force on the rear wall in the closed position. ### Claim 19 A thermal cycler system comprising: a sample block comprising a sample holder receiving surface configured to receive the sample holder; and a cover assembly moveable in a first direction parallel to the sample holder receiving surface between an open position and a closed position relative to the sample holder receiving surface, wherein the cover assembly covers the sample holder receiving surface in the closed position and exposes the sample holder receiving surface in the open position, wherein the cover assembly comprises: a platen moveable, in the closed position of the cover assembly, in a second direction perpendicular to the first direction between a raised position and a lowered position relative to the sample holder receiving surface, a cover lid over the platen, the cover lid comprising a cover surface and opposing side walls extending from the cover surface, an actuatable linkage extending transversely across the platen in a direction perpendicular to the first direction, opposite ends of the actuatable linkage being received in recesses in an interior surface of the opposing side walls of the cover lid, the opposite ends being movable along the recesses in the second direction to move the actuatable linkage in the second direction between a raised position and a lowered position, and an elastically deformable member between the actuatable linkage and the platen, the elastically deformable member arranged to exert a compressive force against the platen in the lowered position of the platen and the actuatable linkage, wherein: the actuatable linkage comprises at least one through-hole extending in the second direction, and the cover lid further comprises at least one guide extending through the at least one through-hole configured to: slide through the at least one through-hole in the second direction, guide movement of the platen in the second direction between the raised position and the lowered position, and minimize movement of the actuatable linkage in the first direction.