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US 11,110,458 B2Granted
System for detection of spaced droplets
AssigneeBio-Rad Laboratories, Inc.
InventorsGeorge Carman, Thomas H. Cauley, III, David P. Stumbo
Life of this patent
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Abstract
System, including methods and apparatus, for spacing droplets from each other and for detection of spaced droplets.
Claims9
A detection system for droplet-based assays, comprising
- a droplet channel including a droplet inlet configured to receive droplets suspended in a carrier fluid;
- a shell surrounding a portion of the droplet channel and having an input channel therein;
- a porous cylinder disposed within the shell and having a central bore that forms a portion of the droplet channel, the porous cylinder having porous walls that provide fluid communication between a periphery of the porous cylinder and the central bore;
- a source of dilution fluid connected to the input channel and disposed outside the shell;
- a detection channel in fluid communication with, and disposed downstream from, the droplet channel;
- a light source to generate light that irradiates the detection channel;
- one or more pumps operable to (a) drive travel of droplets and carrier fluid through the central bore and the detection channel and (b) force flow of dilution fluid into the central bore via the porous walls;
- and
- a detection region including a fluorescence detector and one or more optical elements, the one or more optical elements being configured to direct, to the fluorescence detector, fluorescence radiation emitted by droplets passing through the detection channel.
6 more elements
A detection system for droplet-based assays, comprising
- a droplet channel including a droplet inlet configured to receive droplets suspended in a carrier fluid;
- a shell surrounding a portion of the droplet channel and having an input channel therein;
- an annular frit disposed within the shell and having a central bore that forms a portion of the droplet channel, the annular frit having porous walls that provide fluid communication between a periphery of the annular frit and the central bore;
- a source of dilution fluid connected to the input channel and disposed outside the shell;
- a detection channel in fluid communication with, and disposed downstream from, the droplet channel;
- a light source to generate light that irradiates the detection channel;
- one or more pumps operable to (a) drive travel of droplets and carrier fluid through the central bore and the detection channel and (b) force flow of dilution fluid into the central bore via the porous walls;
- and
- a detection region including a fluorescence detector and one or more optical elements, the one or more optical elements being configured to direct, to the fluorescence detector, fluorescence radiation emitted by droplets passing through the detection channel.
6 more elements
Dependencies are inferred from the claim text.