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US 11,110,457 B2Granted
Analysis unit for a transportable microfluidic device, in particular for sample preparation and molecule analysis
AssigneeSTMicroelectronics S.r.l.
InventorsMarco Cereda, Lillo Raia, Danilo Pirola
Life of this patent
Estimating the term…
Abstract
An analysis unit formed by an analysis body housing an analysis chamber and having a sample inlet and a supply channel configured to fluidically connect the sample inlet to the analysis chamber. Dried assay reagents are arranged in the analysis chamber and are contained in an alveolar mass. For instance, the alveolar mass is a lyophilized mass formed by excipients and by assay-specific reagents.
Claims20
A portable microfluidic device, comprising:
- a cartridge including a first layer, a second layer and a body between the first layer and the second layer, the body having a first side coupled to the first layer, a second side opposite the first side coupled to the second layer and a third side between the first side and the second side having a sample inlet opening, the cartridge including:
- an extraction chamber in fluidic connection with the sample inlet opening, the extraction chamber defined by a first recess in the first side of the body and the first layer;
- a waste chamber in fluidic connection with the extraction chamber, the waste chamber defined by the first layer, a through opening in the body and a corresponding recess in the second layer;
- a collector extending along the extraction chamber and the waste chamber, the collector including an analysis chamber defined by a second recess in the first side of the body and a heating and temperature-control element coupled to the second recess, wherein the heating and temperature-control element is coupled to the first side of the body via a through opening in the first layer;
- and
- a fluidic circuit that couples the extraction chamber, the collector and the waste chamber;
- and
- dried assay reagents in the analysis chamber, wherein the dried assay reagents are part of an alveolar mass.
5 more elements
A method for sample analysis, comprising:
- filling a supply channel of a portable microfluidic device with a sample to be analyzed, the portable microfluidic device including:
- a cartridge including a first layer, a second layer and a body between the first layer and the second layer, the body having a first side coupled to the first layer, a second side opposite the first side coupled to the second layer and a third side between the first side and the second side having a sample inlet opening, the cartridge including:
- an extraction chamber in fluidic connection with the sample inlet opening, the extraction chamber defined by a first recess in the first side of the body and the first layer;
- and
- a waste chamber in fluidic connection with the extraction chamber, the waste chamber defined by the first layer, a through opening in the body and a corresponding recess in the second layer;
- a collector extending along the extraction chamber, the collector including an analysis chamber, wherein the supply channel that couples the extraction chamber and the collector, the analysis chamber defined by a second recess in the first side of the body and a heating and temperature-control element coupled to the second recess, wherein the heating and temperature-control element is coupled to the first side of the body via a through opening in the first layer;
- and
- dried assay reagents in the analysis chamber, wherein the dried assay reagents are part of an alveolar mass;
- and
- causing the alveolar mass to absorb a predetermined amount of the sample to be analyzed in the analysis chamber.
7 more elements
A portable microfluidic device, comprising:
- a casing having a sample inlet opening and housing:
- an extraction chamber in fluidic connection with the sample inlet opening;
- a waste chamber in fluidic connection with the extraction chamber;
- a collector extending along the extraction chamber and the waste chamber, the collector including an analysis unit, the analysis unit comprising:
- a plurality of analysis chambers arranged in an array;
- dried assay reagents in each of the plurality of analysis chambers, wherein the dried assay reagents in each of the plurality of analysis chambers are part of an alveolar mass;
- and
- a supply channel configured to fluidically couple the plurality of analysis chambers to the extraction chamber and the waste chamber, the supply channel having a first branch and a second branch extending in a first direction and a third branch that couples the first branch to the second branch extending in a second direction traversing the first direction, the first branch coupled to a first set of the plurality of analysis chambers and the second branch coupled to a second set of the plurality of analysis chambers;
- and
- a fluidic circuit that couples the extraction chamber with the collector and with the waste chamber.
7 more elements
Dependencies are inferred from the claim text.