# US 11,110,398 B2 · Method for enhancing filtration yields in tangential flow filtration system > Agents: this data is available as JSON from the patentgrep API and MCP server — see https://patentgrep.com/llms.txt - Number: US 11,110,398 B2 - Document ID: US11110398B2 - Status: Granted - Kind: B2 - Application number: 15/750,218 - Filed: 2016-08-17 - Published: 2018-08-09 - Granted: 2021-09-07 - Earliest claimed: 2015-08-20 - Assignees: Cytiva Sweden AB - Applicants: Cytiva Sweden AB - Inventors: Sasi Kumar Nutalapati; Klaus Gebauer; Karl Axel Jakob Liderfelt; Nachiket Karmarkar; Ajit S. Vernekar; Amit Kumar Sharma; Fredrik Oskar Lundstrom; Sangeetha Raghu Ramachandran - Web page: https://patentgrep.com/patent/US11110398B2 ## CPC - B01D 61/18: Apparatus therefor - B01D 61/10: Accessories; Auxiliary operations - B01D 61/12: Controlling or regulating - B01D 61/20: Accessories; Auxiliary operations - B01D 61/22: Controlling or regulating - B01D 65/02: Membrane cleaning or sterilisation {; Membrane regeneration} - C07K 1/34: by filtration, ultrafiltration or reverse osmosis - B01D 2311/08: Specific process operations in the concentrate stream - B01D 2311/16: Flow or flux control - B01D 2311/2512: to feed side - B01D 2313/243: Pumps - B01D 2315/10: Cross-flow filtration - B01D 2315/14: Batch-systems - B01D 2321/02: Forward flushing - B01D: SEPARATION (separating solids from solids by wet methods B03B, B03D; by pneumatic jigs or tables B03B; by other dry methods B07; magnetic or electrostatic separation of solid materials from solid materials or fluids, separation by high-voltage electric fields B03C; centrifuges, vortex apparatus B04; presses per se for squeezing-out liquid from liquid-containing material B30B9/02; treatment of water C02F, e.g. softening by ion-exchange C02F1/42; {arrangements of air intake cleaners in gas turbine plants F02C7/05} ; arrangements or mounting of filters in air-conditioning, air-humidification or ventilation F24F13/28) - C07K: PEPTIDES (peptides in foodstuffs A23; obtaining protein compositions for foodstuffs, working-up proteins for foodstuffs A23J; preparations for medicinal purposes A61K; peptides containing beta-lactam rings C07D; cyclic dipeptides not having in their molecule any other peptide link than those which form their ring, e.g. piperazine-2,5-diones, C07D; ergot alkaloids of the cyclic peptide type C07D519/02; macromolecular compounds having statistically distributed amino acid units in their molecules, i.e. when the preparation does not provide for a specific; but for a random sequence of the amino acid units, homopolyamides and block copolyamides derived from amino acids C08G69/00; macromolecular products derived from proteins C08H1/00; preparation of glue or gelatine C09H; single cell proteins, enzymes C12N; genetic engineering processes for obtaining peptides C12N15/00; compositions for measuring or testing processes involving enzymes C12Q; investigation or analysis of biological material G01N33/00) ## Abstract The disclosure generally relates to methods and apparatus for the efficient quantitative recovery of valuable biological fluids from filtration systems, more particularly to efficient quantitative recovery of valuable biological fluids from high precision separation systems suitable for use in the pharmaceutical and biotechnology industries. ## Claims ### Claim 1 A process for enhanced recovery of a biological fluid from an activated crossflow filtration system, the process comprising: a. inducing an internalized pressurized countercurrent flow throughout the activated crossflow filtration system by activating an internal pump fluidically coupled to a sterile air source at a first end of the activated crossflow filtration system to cause said biological fluid to flow in a flow direction from the sterile air source toward a drain valve and opposite to a crossflow direction, b. recovering said biological fluid at the drain valve fluidically coupled at a second end opposite the first end of the activated crossflow filtration system, wherein the crossflow direction is along a fluid loop from a biofluid source container to a crossflow filtration filter and back to the biofluid source container, wherein the activating of the internal pump causes sterile air from the sterile air source to be introduced into the fluid loop between the biofluid source container and the crossflow filtration filter, and wherein said inducing of the internalized pressurized countercurrent flow comprises supplying sterile air from the sterile air source passively. ### Claim 2 (depends on 1) The process according to claim 1, wherein said internal pump is a peristaltic pump. ### Claim 3 (depends on 1) The process according to claim 1, wherein said activated crossflow filtration system comprises a series of tubes, valves, sensors, and conduits connected together to form a flowpath. ### Claim 4 (depends on 1) The process according to claim 1, wherein said internal pump is located adjacent to the biofluid source container. ### Claim 5 (depends on 1) The process according to claim 1, wherein activating said internal pump comprises operating said internal pump between 10-10,000 rpm. ### Claim 6 (depends on 1) The process according to claim 1, wherein activating said internal pump comprises operating said internal pump between 100-1000 rpm. ### Claim 7 (depends on 1) The process according to claim 1, wherein activating said internal pump comprises operating said internal pump to generate an air flow output of from 1 mL/min to 1 L/min. ### Claim 8 (depends on 7) The process according to claim 7, wherein said internal pump generates pressure between 1 psi to 150 psi. ### Claim 9 (depends on 7) The process according to claim 7, wherein said internal pump generates pressure between 14 to 100 psi. ### Claim 10 (depends on 1) The process according to claim 1, wherein said internal pump generates pressure between 14 to 50 psi. ### Claim 11 (depends on 1) The process according to claim 1 for enhanced recovery of a biological fluid from the activated crossflow filtration system wherein said internal pump induces air flow at about 2 L/min. ### Claim 12 (depends on 1) The process according to claim 1 for enhanced recovery of a biological fluid from the activated crossflow filtration system wherein said induction of the internalized pressurized countercurrent flow is about 25% of a maximum flow rate.