# US 11,110,296 B2 · Bactericidal methods and compositions

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- Number: US 11,110,296 B2
- Document ID: US11110296B2
- Status: Granted
- Kind: B2
- Application number: 17/005,807
- Filed: 2020-08-28
- Published: 2021-03-04
- Granted: 2021-09-07
- Earliest claimed: 2019-01-31
- Assignees: Pulsethera, Inc.
- Applicants: Pulsethera Corporation
- Inventors: Pu-Ting Dong; Jie Hui; Ji-Xin Cheng; Yifan Zhu
- Web page: https://patentgrep.com/patent/US11110296B2

## CPC

- A61K 45/06: Mixtures of active ingredients without chemical characterisation, e.g. antiphlogistics and cardiaca
- A61N 5/062: {Photodynamic therapy, i.e. excitation of an agent}
- A01N 25/00: Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application {, e.g. seed treatment or sequential application}; Substances for reducing the noxious effect of the active ingredients to organisms other than pests
- A01N 59/00: Biocides, pest repellants or attractants, or plant growth regulators containing elements or inorganic compounds
- A61K 31/7036: having at least one amino group directly attached to the carbocyclic ring, e.g. streptomycin, gentamycin, amikacin, validamycin, fortimicins
- A61K 31/7048: having oxygen as a ring hetero atom, e.g. leucoglucosan, hesperidin, erythromycin, nystatin {, digitoxin or digoxin}
- A61K 33/38: Silver; Compounds thereof
- A61K 33/40: Peroxides
- A61L 2/10: Ultraviolet [UV] radiation
- A61L 2/208: Hydrogen peroxide
- A61N 5/0616: {Skin treatment other than tanning}
- A61N 5/0624: {for eliminating microbes, germs, bacteria on or in the body}
- A61N 5/067: using laser light
- A61L 2101/02: Inorganic materials
- A61L 2103/50: Textiles, e.g. bedwear or towels
- A61N 2005/0635: {characterised by the body area to be irradiated}
- A61N 2005/0643: {Applicators, probes irradiating specific body areas in close proximity}
- A61N 2005/0651: {Diodes}
- A61N 2005/0662: {Visible light}
- A61K: PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES (devices or methods specially adapted for bringing pharmaceutical products into particular physical or administering forms A61J3/00; chemical aspects of, or use of materials for deodorisation of air, for disinfection or sterilisation, or for bandages, dressings, absorbent pads or surgical articles A61L; soap compositions C11D)
- A61N: ELECTROTHERAPY; MAGNETOTHERAPY; RADIATION THERAPY; ULTRASOUND THERAPY (measurement of bioelectric currents A61B; surgical instruments, devices or methods for transferring non-mechanical forms of energy to or from the body A61B18/00; anaesthetic apparatus in general A61M; incandescent lamps H01K; infrared radiators for heating H05B)
- A01N: PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF (preservation of food or foodstuff A23); BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES (preparations for medical, dental or toiletry purposes which kill or prevent the growth or proliferation of unwanted organisms A61K); PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A61L: METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES (preservation of bodies or disinfecting characterised by the agents employed A01N; preserving, e.g. sterilising, food or foodstuffs A23; preparations for medical, dental or toiletry purposes A61K)

## Abstract

Methods of the present invention comprise photoinactivation of catalase in combination with low-concentration peroxide solutions and/or ROS generating agents to provide antibacterial effects.

## Claims

### Claim 1

A method of disinfecting an inanimate surface contaminated with a catalase-positive microbe, said method comprising the steps of: applying pulsed visible light to the inanimate surface at a wavelength of 405 nm to 420 nm and a dose of about 5 J/cm2 or greater, wherein the catalase is inactivated, and subsequently contacting the inanimate surface with a composition comprising a diluted peroxide solution, thereby disinfecting the inanimate surface.

### Claim 2 (depends on 1)

The method of claim 1, wherein the wavelength is 410 nm.

### Claim 3 (depends on 1)

The method of claim 1, wherein the dose of the light is about 15 J/cm2.

### Claim 4 (depends on 1)

The method of claim 1, wherein the catalase-positive microbe is a fungus or bacteria and the light is provided by a pulsed nanosecond laser.

### Claim 5 (depends on 1)

The method of claim 1, wherein the diluted peroxide solution is a hydrogen peroxide solution.

### Claim 6 (depends on 5)

The method of claim 5, wherein the hydrogen peroxide solution is between about 0.03% and about 0.3% hydrogen peroxide.

### Claim 7 (depends on 5)

The method of claim 5, wherein the wherein the hydrogen peroxide solution is 0.3% hydrogen peroxide.

### Claim 8 (depends on 1)

The method of claim 1, wherein the inanimate surface is a material comprising metal, plastic, fabric, rubber, stone, composite surfaces or wood.

### Claim 9 (depends on 1)

The method of claim 1, wherein the dose of the light is about 5 J/cm2 to about 200 J/cm2.