Recombinant Zika vaccines

An infectious replicative virus particle comprising a measles virus vector backbone from one of the Schwarz strain, the Zagreb strain, the AIK-C strain and the Moraten strain, and a Zika virus E-protein, wherein the infectious replicative virus particle comprises in its genome at least two nucleic acid sequences, wherein (i) the first nucleic acid sequence encodes at least one E-protein of a Zika virus;

• and (ii) the second nucleic acid sequence encodes the measles virus vector backbone;
• wherein:
• (a) the first nucleic acid sequence is operably linked to the second nucleic acid sequence;
• (b) the first nucleic acid sequence comprises, in sequential order:
• (1) a nucleic acid sequence encoding a capsid cleavage site, the capsid cleavage site comprising the residues arginine-arginine immediately preceding the nucleic acid sequence of (2) below;
• (2) a nucleic acid sequence encoding at least one pre-membrane protein signal sequence of a Zika virus pre-membrane protein;
• (3) a nucleic acid sequence encoding a pre-membrane protein of a Zika virus;
• (4) a nucleic acid sequence encoding an E-protein signal sequence of a Zika virus;
• and
• (5) a nucleic acid sequence encoding the at least one E-protein of a Zika virus, the nucleic acid sequence encoding at least one E-protein of a Zika virus comprising the E-protein portion encoded by any one of SEQ ID NOs:
• 1 to 13 and 67 to 87, wherein the nucleic acid sequence encoding the at least one E-protein of a Zika virus does not comprise a sequence encoding a stem-anchor region, and wherein the nucleic acid sequence encoding the at least one E-protein of a Zika virus encodes a mutation L107D in comparison to the sequence of any one of SEQ ID NOs:40 to 52 or 130 to 150;
• and
• (c) the infectious replicative virus particle does not comprise a nucleic acid sequence encoding a C-protein or a non-structural protein of a flavivirus.

An infectious replicative virus particle comprising a measles virus vector backbone from one of the Schwarz strain, the Zagreb strain, the AIK-C strain and the Moraten strain, and a Zika virus E-protein, wherein the infectious replicative virus particle comprises in its genome at least two nucleic acid sequences, wherein (i) the first nucleic acid sequence encodes at least one E-protein of a Zika virus;

• and (ii) the second nucleic acid sequence encodes the measles virus vector backbone;
• wherein:
• (a) the first nucleic acid sequence is operably linked to the second nucleic acid sequence;
• (b) the first nucleic acid sequence comprises, in sequential order:
• (1) a nucleic acid sequence encoding a capsid cleavage site, the capsid cleavage site comprising the residues arginine-arginine immediately preceding the nucleic acid sequence of (2) below;
• (2) a nucleic acid sequence encoding at least one pre-membrane protein signal sequence of a Zika virus pre-membrane protein;
• (3) a nucleic acid sequence encoding a pre-membrane protein of a Zika virus;
• (4) a nucleic acid sequence encoding an E-protein signal sequence of a Zika virus;
• and
• (5) a nucleic acid sequence encoding the at least one E-protein of a Zika virus, the nucleic acid sequence encoding at least one E-protein of a Zika virus comprising the E-protein portion encoded by any one of SEQ ID NOs:
• 1 to 13 and 67 to 87, wherein the nucleic acid sequence encoding the at least one E-protein of a Zika virus does not comprise a sequence encoding a stem-anchor region;
• and
• (c) the infectious replicative virus particle does not comprise a nucleic acid sequence encoding a C-protein or a non-structural protein of a flavivirus.

An infectious replicative virus particle comprising a measles virus vector backbone from one of the Schwarz strain, the Zagreb strain, the AIK-C strain and the Moraten strain, and a Zika virus E-protein, wherein the infectious replicative virus particle comprises in its genome at least two nucleic acid sequences, wherein (i) the first nucleic acid sequence encodes at least one E-protein of a Zika virus;

• and (ii) the second nucleic acid sequence encodes the measles virus vector backbone;
• wherein:
• (a) the first nucleic acid sequence is operably linked to the second nucleic acid sequence;
• (b) the first nucleic acid sequence comprises, in sequential order:
• (1) a nucleic acid sequence encoding a capsid cleavage site, the capsid cleavage site comprising the residues arginine-arginine immediately preceding the nucleic acid sequence of (2) below;
• (2) a nucleic acid sequence encoding at least one pre-membrane protein signal sequence of a Zika virus pre-membrane protein;
• (3) a nucleic acid sequence encoding a pre-membrane protein of a Zika virus;
• (4) a nucleic acid sequence encoding an E-protein signal sequence of a Zika virus;
• and
• (5) a nucleic acid sequence encoding the at least one E-protein of a Zika virus, the nucleic acid sequence encoding at least one E-protein of a Zika virus comprising the E-protein portion encoded by any one of SEQ ID NOs:
• 1 to 13 and 67 to 87, wherein the nucleic acid sequence encoding the at least one E-protein of a Zika virus comprises a sequence encoding a stem-anchor region of an E-protein of a Zika virus, or a heterologous stem-anchor region, and wherein the nucleic acid sequence encoding the at least one E-protein of a Zika virus encodes a mutation L107D in comparison to the sequence of any one of SEQ ID NOs:40 to 52 or 130 to 150;
• and
• (c) the infectious replicative virus particle does not comprise a nucleic acid sequence encoding a C-protein or a non-structural protein of a flavivirus.

An infectious replicative virus particle comprising a measles virus vector backbone from one of the Schwarz strain, the Zagreb strain, the AIK-C strain and the Moraten strain, and a Zika virus E-protein, wherein the infectious replicative virus particle comprises in its genome at least two nucleic acid sequences, wherein (i) the first nucleic acid sequence encodes at least one E-protein of a Zika virus;

• and (ii) the second nucleic acid sequence encodes the measles virus vector backbone;
• wherein:
• (a) the first nucleic acid sequence is operably linked to the second nucleic acid sequence;
• (b) the first nucleic acid sequence comprises, in sequential order:
• (1) a nucleic acid sequence encoding a capsid cleavage site, the capsid cleavage site comprising the residues arginine-arginine immediately preceding the nucleic acid sequence of (2) below;
• (2) a nucleic acid sequence encoding at least one pre-membrane protein signal sequence of a Zika virus pre-membrane protein;
• (3) a nucleic acid sequence encoding a pre-membrane protein of a Zika virus;
• (4) a nucleic acid sequence encoding an E-protein signal sequence of a Zika virus;
• and
• (5) a nucleic acid sequence encoding the at least one E-protein of a Zika virus, the nucleic acid sequence encoding at least one E-protein of a Zika virus comprising the E-protein portion encoded by any one of SEQ ID NOs:
• 1 to 13 and 67 to 87, wherein the nucleic acid sequence encoding the at least one E-protein of a Zika virus comprises a sequence encoding a stem-anchor region of an E-protein of a Zika virus, or a heterologous stem-anchor region;
• and
• (c) the infectious replicative virus particle does not comprise a nucleic acid sequence encoding a C-protein or a non-structural protein of a flavivirus.