# US 11,110,139 B2 · Application of anthocyanin extract in preparing pharmaceutical composition for preventing and treating cardiac toxicity induced by anthracyclines and pharmaceutical composition

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- Number: US 11,110,139 B2
- Document ID: US11110139B2
- Status: Granted
- Kind: B2
- Application number: 15/831,411
- Filed: 2017-12-05
- Published: 2019-01-03
- Granted: 2021-09-07
- Earliest claimed: 2017-06-30
- Assignees: Northwest Institute of Plateau Biology, Chinese Academy of Sciences
- Applicants: Northwest Institute of Plateau Biology, Chinese Academy of Sciences
- Inventors: Tingting Gao; Hongtao Bi; Lixin Wei; Yuzhi Du; Hongxia Yang; Cen Li
- Web page: https://patentgrep.com/patent/US11110139B2

## CPC

- A61K 36/185: Magnoliopsida (dicotyledons)
- A61P 9/00: Drugs for disorders of the cardiovascular system
- A61K 2236/333: using mixed solvents, e.g. 70% EtOH
- A61K: PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES (devices or methods specially adapted for bringing pharmaceutical products into particular physical or administering forms A61J3/00; chemical aspects of, or use of materials for deodorisation of air, for disinfection or sterilisation, or for bandages, dressings, absorbent pads or surgical articles A61L; soap compositions C11D)
- A61P: SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS

## Abstract

The disclosure provides an application of an anthocyanin extract in preparing a pharmaceutical composition for preventing and treating cardiac toxicity induced by Anthracyclines and the pharmaceutical composition, wherein the pharmaceutical composition comprises an effective dose of the anthocyanin extract; and the anthocyanin extract refers to an extract obtained from mature fruits of a zygophyllaceous nitraria plant and having a total anthocyanin content greater than 700 mg CGE/g and a total antioxidant capacity greater than 200 mg TE/g. Cytobiology and molecular biology studies prove that the anthocyanin extract has obvious protection effect to cardiomyocyte toxicity damage induced by anthracyclines and may be used for preventing and alleviating cardiotoxicity of an antharcyclines anticancer drug, thereby expanding a clinical application of the antharcyclines anticancer drug and relieving toxic and side effects brought by the antharcyclines anticancer drug to a patient and pain of a chemotherapy patient. And additionally, the raw material sources for the anthocyanin extract are wide, the cost is low and the use is safe.

## Claims

### Claim 1

A method for preventing and treating cardiac toxicity induced by Anthracyclines, said method comprising administering a pharmaceutical composition comprising an effective dose of an anthocyanin extract to a subject in need thereof, and the anthocyanin extract is obtained from mature fruits of a zygophyllaceous nitraria plant and having a total anthocyanin content greater than 700 mg CGE/g and a total antioxidant capacity greater than 200 mg TE/g; wherein an anthocyanidin as a source of the anthocyanin extract comprises cyanidin, delphinidin, malvidin, pelargonidin, peonidin and petunidin.

### Claim 2 (depends on 1)

The method according to claim 1, wherein the anthocyanin extract comprises six or more selected from groups composed of cyanidin-3-O-diglucoside, cyanidin-3-O-sambubioside, cyanidin-3-O-(cis-6″-O-coumaroyl)-diglucoside, cyanidin-3-O-(trans-6″-O-coumaroyl)-diglucoside, cyanidin-3-O-(6″-O-coumaroyl)-glucoside, delphinidin-3-O-(6″-O-coumaroyl)-glucoside, 5-O-glucoside, delphinidin-3-O-(6″-O-coumaroyl)-glucoside, malvidin-3-O-glucoside, malvidin-3-O-(6″-O-acetyl)-glucoside, malvidin-3-O-(6″-O-coumaroyl)-glucoside, 5-O-glucoside, malvidin-3-O-(cis-6″-O-coumaroyl)-glucoside, malvidin-3-O-(trans-6″-O-coumaroyl)-glucoside, pelargonidin-3-O-(6″-O-coumaroyl)-diglucoside, peonidin-3-O-(6″-O-coumaroyl)-glucoside, peonidin-3-O-(6″-O-coumaroyl)-glucoside, 5-O-glucoside and petunidin-3-O-(6″-O-coumaroyl)-glucoside; and the anthocyanidin as the source of the anthocyanin extract comprises the cyanidin, the delphinidin, the malvidin, the pelargonidin, the peonidin and the petunidin.

### Claim 3 (depends on 1)

The method according to claim 1, wherein the anthocyanin extract is prepared by the following method: adding 60-90% ethanol to pomaces, which are produced by nitraria fruits or after the nitraria fruits are squeezed into juice, according to a solid-liquid ratio of 1:15-1:30 g/mL, and extracting for 30-90 min at 55-65° C.; concentrating an extracting solution under a reduced pressure till there is no ethanol, separating by a weakly polar macroporous adsorption resin, rinsing with three times of water by a column volume, then rinsing with the 60-90% ethanol, collecting an effluent, concentrating and drying to obtain the anthocyanin extract.

### Claim 4 (depends on 1)

The method according to claim 1, wherein the anthracyclines are one or more of doxorubicin (DOX), daunorubicin (DNR), aclacinomycin, epirubicin, pirarubicin, idarubicin, valrubicin or mitoxantrone.

### Claim 5 (depends on 1)

The method according to claim 1, wherein a dosage form of the pharmaceutical composition is any one of a capsule, a tablet, an oral liquid, a granule and a dropping pill.