# US 11,110,134 B2 · Direct-fed microbials

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- Number: US 11,110,134 B2
- Document ID: US11110134B2
- Status: Granted
- Kind: B2
- Application number: 16/454,593
- Filed: 2019-06-27
- Published: 2020-01-16
- Granted: 2021-09-07
- Earliest claimed: 2016-02-29
- Assignees: Microbial Discovery Group, LLC; United Animal Health, Inc.
- Applicants: Microbial Discovery Group, LLC; United Animal Health, Inc.
- Inventors: Michael R. King; Sona Son; Kyle Leistikow; Nathan Robert Augspurger
- Web page: https://patentgrep.com/patent/US11110134B2

## CPC

- A61K 35/742: Spore-forming bacteria, e.g. Bacillus coagulans, Bacillus subtilis, clostridium or Lactobacillus sporogenes
- A23K 10/18: of live microorganisms
- A23K 20/10: Organic substances
- A23K 20/195: Antibiotics
- A23K 50/30: for swines
- A23K 50/75: for poultry
- A61K 35/74: Bacteria (therapeutic use of a bacterial protein A61K38/00)
- A61K 38/43: Enzymes; Proenzymes; Derivatives thereof
- A61K 45/06: Mixtures of active ingredients without chemical characterisation, e.g. antiphlogistics and cardiaca
- A61P 31/04: Antibacterial agents
- A61K: PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES (devices or methods specially adapted for bringing pharmaceutical products into particular physical or administering forms A61J3/00; chemical aspects of, or use of materials for deodorisation of air, for disinfection or sterilisation, or for bandages, dressings, absorbent pads or surgical articles A61L; soap compositions C11D)
- A23K: FODDER
- A61P: SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS

## Abstract

The invention relates to direct-fed microbials for use in E. coli inhibition in animals. More particularly, the invention relates to isolated Bacillus strains 101, 235, 77, 177, and 102 and strains having all of the identifying characteristics of these strains, for a use comprising the above-mentioned use. The invention can also be used for treatment of plants and in food processing.

## Claims

### Claim 1

A nutrient composition comprising an isolated Bacillus strain selected from the group consisting of Bacillus strain 101 (NRRL No. B-67218), Bacillus strain 235 (NRRL No. B-67219), Bacillus strain 77 (NRRL No. B-67274), Bacillus strain 177 (NRRL No. B-67275), Bacillus strain 102 (NRRL No. B-67276), a strain having all of the identifying characteristics of Bacillus strain 101 (NRRL No. B-67218), a strain having all of the identifying characteristics of Bacillus strain 235 (NRRL No. B-67219), a strain having all of the identifying characteristics of Bacillus strain 77 (NRRL No. B-67274), a strain having all of the identifying characteristics of Bacillus strain 177 (NRRL No. B-67275), a strain having all of the identifying characteristics of Bacillus strain 102 (NRRL No. B-67276), and combinations thereof, wherein the strain(s) are in a form selected from the group consisting of a pellet, a gel, a freeze-dried composition, a top-dressing, a liquid, and a dried powder.

### Claim 2 (depends on 1)

The composition of claim 1 wherein the composition further comprises a carrier.

### Claim 3 (depends on 1)

The composition of claim 1 wherein the composition further comprises an exogenously added nutrient component selected from the group consisting of a vitamin, an antibiotic, an enzyme, a water-soluble or water-insoluble monosaccharide, disaccharide, or polysaccharide, a fat, phosphorous, sodium bicarbonate, limestone, calcium, sodium, sulfur, magnesium, potassium, copper, iron, manganese, zinc, fish oil, raw seed, an antioxidant, and a starch.

### Claim 4 (depends on 1)

The composition of claim 1 further comprising a binder wherein the binder is selected from the group consisting of clay, yeast cell wall components, aluminum silicate, glucan, and combinations thereof.

### Claim 5 (depends on 1)

The composition of claim 1 in the form of a commercial package.

### Claim 6 (depends on 5)

The composition of claim 5 wherein the commercial package comprises a container selected from the group consisting of a bag, a pouch, a drum, a bottle, and a box.

### Claim 7 (depends on 2)

The composition of claim 2 wherein the carrier is selected from the group consisting of a bran, rice hulls, a salt, mineral oil, a dextrin, whey, sugar, limestone, dried starch, sodium silico aluminate, vegetable oil, and combinations thereof.

### Claim 8 (depends on 3)

The composition of claim 3 wherein the exogenously added component is an enzyme.

### Claim 9 (depends on 8)

The composition of claim 8 wherein the enzyme is selected from the group consisting of a galactosidase, a protease, a lipase, an amylase, a hemicellulase, an arabinoxylanase, a xylanase, a cellulase, an NSPase, a phytase, and combinations thereof.