Lyophilized composition for preserving microbiota in its ecosystem
AssigneesInstitut National De Recherche Pour L'agriculture, L'alimentation Et L'environnement, Universite Paris Descartes, Universite Paris Diderot—Paris 7, Assistance Publique-Hopitaux de Paris
InventorsNathalie Kapel, Anne-Judith Waligora-Dupriet, Muriel Thomas, Christine Charrueau, Francisca Joly, Camille Mayeur, Véronique Robert, Johanne Delannoy
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Abstract
The invention relates to a lyophilized composition comprising a microbiota in its ecosystem, in particular stools, and a mixture of cryoprotectant agents comprising maltodextrin and trehalose, a capsule comprising said lyophilized composition, the use thereof, and methods for the production thereof.
Claims11
A lyophilized composition comprising a collected microbiota and a mixture of cryoprotectants,
- wherein said mixture of cryoprotectants comprises maltodextrin and trehalose in a maltodextrin/trehalose (M/T) weight ratio ranging from 35/65 to 45/55,
- wherein said microbiota is faecal microbiota contained in stool, and
- wherein said lyophilized composition comprises at least one viable bacterial population that show extreme oxygen sensitivity (EOS) after at least 6 months of storage and wherein said microbiota is unchanged compared with the microbiota existing naturally in the body and further comprises at least bacterial metabolites.
A method for preserving a microbiota in a lyophilized form, comprising:
- contacting the microbiota with a mixture of cryoprotectants, wherein said mixture of cryoprotectants comprises maltodextrin (M) and trehalose (T) in an M/T weight ratio ranging between 35/65 and 45/55;
- and
- lyophilizing the microbiota wherein the microbiota is faecal microbiota contained in stool.
Dependencies are inferred from the claim text.