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US 11,105,823 B2Granted
Methods and compositions for identifying non-alcoholic fatty liver disease
AssigneeThe Regents of the University of California
InventorsEdward A. Dennis, Oswald Quehenberger, Rohit Loomba
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Abstract
The disclosure provides methods for differentiating between non-alcoholic fatty liver disease and non-alcoholic steatohepatitis. The method includes measuring eicosanoids and fatty acid levels in a biological sample.
Claims7
A method of differentiating nonalcoholic steatohepatitis (NASH) from nonalcoholic fatty liver (NAFL) in a subject, comprising:
- (a) obtaining a biological sample from the subject;
- (b) treating the sample with 0.6-0.7M KOH to release esterified eicosanoids and recovering the eicosanoids and polyunsaturated fatty acids (PUFAs);
- (c) measuring the level of (i) 8-HETrE;
- (ii) 15-HETrE, (iii) omega-3 fatty acid DHA, and (iv) 17-HDoHE, and optionally one or more additional eicosanoids and/or PUFAs selected from the group consisting of 5-HETE, 8-HETE, 11-HETE, 15-HETE, 13-NODE, and 9-oxoODE;
- (d) expressing the levels of the eicosanoids and PUFAs as the area under receiver operating characteristic curve (AUROC) based upon a ratio of the levels of the eicosanoid and/or PUFAs matched with deuterated internal standards of the same metabolite;
- and
- (e) comparing the expressed levels of eicosanoid and PUFAs to a control sample obtained from a NAFL subject, wherein a difference in the expressed levels of the eicosanoid and PUFAs of at least 0.8 in the sample obtained from the subject compared to the control is indicative of NASH.
4 more elements
Dependencies are inferred from the claim text.