Engineering of acetyl-CoA metabolism in yeast
AssigneeBiopetrolia AB
InventorsJens Nielsen, Verena Siewers, Anastasia Krivoruchko, Yiming Zhang, Zongjie Dai
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Abstract
The invention relates to engineering of acetyl-CoA metabolism in yeast and in particular to production of acetyl-CoA in a non-ethanol producing yeast lacking endogenous gene(s) encoding pyruvate decarboxylase and comprising a heterologous pathway for synthesis of cytosolic acetyl-CoA.
Claims15
A yeast comprising at least one heterologous pathway for synthesis of cytosolic acetyl-Coenzyme A (CoA), the at least one heterologous pathway comprising at least one heterologous gene encoding a respective enzyme involved in synthesis of acetyl-CoA, wherein the yeast lacks any endogenous gene encoding pyruvate decarboxylase or comprises disrupted gene or genes encoding pyruvate decarboxylase, and the at least one heterologous gene comprises:
- a heterologous gene encoding a pyruvate ferredoxin oxidoreductase;
- a heterologous gene encoding a ferredoxin/flavodoxin reductase;
- and
- a heterologous gene encoding a ferredoxin/flavodoxin reductase substrate, wherein the ferredoxin/flavodoxin reductase substrate is ferredoxin and/or flavodoxin.
A method of producing a yeast suitable for the production of cytosolic acetyl-Coenzyme A (CoA) comprising:
- introducing into said yeast at least one heterologous pathway for synthesis of cytosolic acetyl-CoA, said at least one heterologous pathway comprising heterologous genes encoding an enzyme involved in synthesis of acetyl-CoA, with the proviso that said heterologous genes are not a heterologous gene encoding a pyruvate formate lyase,
- wherein the heterologous genes comprise:
- a heterologous gene encoding a pyruvate ferredoxin oxidoreductase;
- a heterologous gene encoding a ferredoxin/flavodoxin reductase;
- and/or
- a heterologous gene encoding a ferredoxin/flavodoxin reductase substrate, wherein the ferredoxin/flavodoxin reductase substrate is ferredoxin and/or flavodoxin;
- and deleting or disrupting any endogenous gene encoding pyruvate decarboxylase (PDC) in said yeast.
Dependencies are inferred from the claim text.