Antigen specific Tregs and related compositions, methods and systems
AssigneeCalifornia Institute of Technology
InventorsJune L. Round, Sarkis K. Mazmanian
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Abstract
Antigen specific regulatory T cells are described and related compositions, methods and systems. Methods to generate an antigen specific anti-inflammatory regulatory T cell is provided, the method comprising contacting either a T cell or an antigen presenting cell with a zwitterionic polysaccharide conjugated to the antigen for a time and under condition to generate an antigen specific regulatory T cell that is capable of inhibiting a pro-inflammatory response against the antigen.
Claims19
A method to generate an antigen specific anti-inflammatory regulatory T cell, the method comprising:
- contacting an antigen presenting cell (APC) with a zwitterionic polysaccharide conjugated to the antigen in vitro in absence of a regulatory T cell to generate an APC presenting the antigen, and thereafter
- contacting the APC presenting the antigen with the regulatory T cell in vivo to generate an antigen specific regulatory T cell capable of inhibiting a pro-inflammatory response against the antigen,
- wherein the zwitterionic polysaccharide is selected from polysaccharide A (PSA) and polysaccharide B (PSB) from B. fragilis and the APC is a dendritic cell.
A method to generate an antigen specific anti-inflammatory regulatory T cell, the method comprising:
- contacting an antigen presenting cell (APC) with a zwitterionic polysaccharide conjugated to the antigen in vitro in absence of a regulatory T cell to generate an APC presenting the antigen, and thereafter
- contacting the APC presenting the antigen with the regulatory T cell to generate an antigen specific regulatory T cell capable of inhibiting a pro-inflammatory response against the antigen,
- wherein the zwitterionic polysaccharide is selected from polysaccharide A (PSA) and polysaccharide B (PSB) from B. fragilis and the APC is a dendritic cell and
- wherein the zwitterionic polysaccharide is conjugated to the antigen by direct or indirect covalent linkage.
A method to generate an antigen specific anti-inflammatory regulatory T cell, the method comprising:
- contacting an antigen presenting cell (APC) with a zwitterionic polysaccharide conjugated to the antigen in vitro in absence of a regulatory T cell to generate an APC presenting the antigen, and thereafter
- contacting the APC presenting the antigen with the regulatory T cell to generate an antigen specific regulatory T cell capable of inhibiting a pro-inflammatory response against the antigen,
- wherein the zwitterionic polysaccharide is selected from polysaccharide A (PSA) and polysaccharide B (PSB) from B. fragilis and the APC is a dendritic cell,
- wherein the antigen is associated with a condition selected from the group consisting of rheumatoid arthritis, myocarditis, scleroderma, type I diabetes, multiple sclerosis, Crohn's disease, ulcerative colitis, Sjorgens syndrome, Hashimoto's thyroiditis, Graves Disease, autoimmune hepatitis, and myasthenia gravis, and
- wherein the antigen is a self-antigen.
Dependencies are inferred from the claim text.