Cells expressing parathyroid hormone 1 receptor and uses thereof
AssigneeTithon Biotech Inc.
InventorVasilis Paspaliaris
Life of this patent
Estimating the term…
Abstract
Disclosed herein are pluripotent stem cells cultured with one or more peptide and methods of isolating said stem cells. Also disclosed are methods of targeting the stem cells to a desired region or area within an organism. Also disclosed are methods of using the isolated stem cells for the improvement of fertility, for the promotion of hair growth, for the treatment or prevention of skin conditions, for the treatment or improvement of bone disorders, for the treatment of malignancies, and for the treatment of neurological disorders.
Claims10
A method of treating an age-related disorder in a mammalian subject in need thereof comprising:
- isolating a population of parathyroid hormone (PTH) receptor-positive pluripotent stem cells from peripheral blood of the subject, wherein the isolated population of PTH receptor-positive pluripotent stem cells express parathyroid hormone type 1 receptor (PTH1R), and the cell population is positive for CD90 and CD133;
- positive/negative for CD29, CD34, CD105, and CD106;
- and negative for SSEA-3, CD200, and CD45, wherein the isolating of PTH receptor-positive stem cells comprises:
- contacting the peripheral blood sample from the subject with an extracorporeal device;
- and
- capturing the PTH receptor-positive pluripotent stem cells on a porous membrane of the extracorporeal device, wherein capturing comprises applying centrifugal force to the extracorporeal device, or wherein the porous membrane comprises an antibody or portion thereof that specifically binds to the PTH receptor-positive pluripotent stem cells;
- transfecting said isolated population of PTH receptor-positive pluripotent stem cells with a human telomerase reverse transcriptase (hTERT) gene to generate hTERT transfected PTH receptor-positive pluripotent stem cells;
- and
- administering the hTERT transfected PTH receptor-positive pluripotent stem cells to said subject in a therapeutically effective amount and via a route sufficient to engraft at least a fraction of the hTERT transfected PTH receptor-positive pluripotent stem cells into the tissue to be treated.
Dependencies are inferred from the claim text.