Fast thermo-optical particle characterisation
AssigneeNanotemper Technologies GmbH
InventorsStefan Duhr, Philipp Baaske
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Abstract
The present invention relates to a method and an apparatus for a fast thermo-optical characterisation of particles. In particular, the present invention relates to a method and a device to measure the stability of (bio)molecules, the interaction of molecules, in particular biomolecules, with, e.g. further (bio)molecules, particularly modified (bio)molecules, particles, beads, and/or the determination of the length/size (e.g. hydrodynamic radius) of individual (bio)molecules, particles, beads and/or the determination of length/size (e.g. hydrodynamic radius).
Claims24
A method of thermo-optically measuring characteristics of a biological cell in a solution comprising:
- providing a sample comprising a marked biological cell in a solution;
- exciting fluorescently said marked biological cell and firstly detecting fluorescence of said excited biological cell;
- irradiating a laser light beam into the solution to obtain a spatial temperature distribution in the solution around the irradiated laser light beam;
- detecting secondly a fluorescence of the biological cell in the solution at a predetermined time within the range of 1 ms to 250 ms after irradiation of the laser into the solution has been started, and characterizing the biological cell based on said two detections.
Dependencies are inferred from the claim text.