ATP phosphoribosyltransferase variant and method for producing L-histidine using the same
AssigneeCj Cheiljedang Corporation
InventorsMyung Keun Park, Nara Kwon, Jin Nam Lee
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Abstract
The present disclosure relates to an ATP phosphoribosyltransferase (HisG) protein and a method for producing histidine using the same.
Claims15
An ATP phosphoribosyltransferase variant having a substitution of an asparagine at position corresponding to 215 with arginine, a substitution of glycine at position corresponding to 233 with a histidine, and a substitution of a threonine at position corresponding to 235 with glutamine in the amino acid sequence of SEQ ID NO:
- 13, wherein the variant comprises an amino acid sequence having at least 95% sequence identity to SEQ ID NO:
- 13, wherein the ATP phosphoribosyltransferase variant has ATP phosphoribosyltransferase activity.
A polynucleotide encoding an ATP phosphoribosyltransferase variant, wherein the ATP phosphoribosyltransferase variant has a substitution of an asparagine at position corresponding to 215 with arginine, a substitution of glycine at position corresponding to 233 with a histidine, and a substitution of a threonine at position corresponding to 235 with glutamine in the amino acid sequence of SEQ ID NO:
- 13, and the variant comprises an amino acid sequence having at least 95% sequence identity to SEQ ID NO:
- 13, wherein the ATP phosphoribosyltransferase variant has ATP phosphoribosyltransferase activity.
A microorganism of the genus Corynebacterium, wherein the microorganism comprises at least one selected from the group consisting of
- (a) an ATP phosphoribosyltransferase variant;
- (b) a polynucleotide encoding the ATP phosphoribosyltransferase variant;
- and
- (c) a vector comprising the polynucleotide encoding the ATP phosphoribosyltransferase variant,
- wherein the ATP phosphoribosyltransferase variant has a substitution of an asparagine at position corresponding to 215 with arginine, a substitution of glycine at position corresponding to 233 with a histidine, and a substitution of a threonine at position corresponding to 235 with glutamine in the amino acid sequence of SEQ ID NO:
- 13, and the variant comprises an amino acid sequence having at least 95% sequence identity to SEQ ID NO:
- 13, and the ATP phosphoribosyltransferase variant has ATP phosphoribosyltransferase activity.
A microorganism transformed with a polynucleotide encoding an ATP phosphoribosyltransferase variant consisting of the amino acid sequence of SEQ ID NO:
- 1.
Dependencies are inferred from the claim text.