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US 11,098,315 B2Granted
Biosynthetic production of steviol glycosides and processes therefore
AssigneeConagen Inc.
InventorsGuohong Mao, Jacob Edward Vick, Michael Batten, Yang Luo, Yilin Wu, Beihua Zhang, David Byun, Xiaodan Yu
Life of this patent
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Abstract
The present invention relates to the production of steviol glycoside rebaudiosides D4, WB1 and WB2 and the production of rebaudioside M from Reb D4.
Claims49
A recombinant cell transformed with a polynucleotide that encodes a mutant of an enzyme having the amino acid sequence of SEQ ID NO:
- 3, wherein the mutant comprises an amino acid sequence having at least 90% sequence identity to the amino acid sequence of SEQ ID NO:
- 3, comprises at least one mutation at an amino acid residue position corresponding to a position selected from 3, 6, 90, 91, 93, 181, 183, 184, 185, 350, 389, 410, 418, 450, 451, 452, and 454 of SEQ ID NO:
- 3, wherein the mutant is a UDP-glycosyltransferase capable of converting rebaudioside D4 having the structure:
- to rebaudioside M.
1 more element
Dependencies are inferred from the claim text.